Colletotrichum fructicola CfGti1通过转录调节果上的透,殖民和病原性
Wenkui Liu1, Wei Zhang2,3, Wenxin Shi1
1Shanxi Center for Testing of Functional Agro-Products, Shanxi Agricultural University, Taiyuan 030031, China.
Journal of fungi (Basel, Switzerland)
|January 27, 2026
概括
转录因子CfGti1对于果中Glomerella叶斑 (GLS) 的发展至关重要. 在Collettotrichum fructicola中删除CfGti1基因可以防止宿主透,并减少真菌殖民,突出其在果病病原体中的作用.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 由Collettotrichum fructicola引起的Glomerella叶斑 (GLS) 显著影响果的生产.
- 导致GLS病变的分子机制在很大程度上是未知的.
- 之前的研究发现,转录因子CfGti1在果叶感染期间被诱导.
研究的目的:
- 为了阐明转录因子CfGti1在果Glomerella叶斑的发病过程中的功能.
- 研究CfGti1在宿主透,真菌生长和毒素产生中的作用.
主要方法:
- 使用同源重组的基因删除突变 (ΔCfGti1) 生成.
- 对果叶子和水果的病原性进行表型分析.
- 进行比较的转录组和qRT-PCR分析以确定受调节的基因.
主要成果:
- 在果叶上,ΔCfGti1突变完全丧失了病原性,并阻断了以压力管为媒介的宿主透.
- 在受伤的果果中,殖民和枝延伸在ΔCfGti1突变中显著减少.
- CfGti1调节了参与压缩性脂滴代谢和宿主特异性毒素 (AK和AF) 生物合成的基因.
结论:
- CfGti1是一个关键的调节器,对于Glomerella叶斑在Colletotrichum fructicola的病变产生至关重要.
- CfGti1在经由appressorium介导的宿主透和真菌发育中发挥着至关重要的作用.
- 这些发现提供了关于果GLS分子基础的见解,以及疾病管理的潜在目标.
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