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细胞粘附和细胞毒性评估与银纳米粒子功能化的原涂层电PCL膜
Chiara Goisis1,2, Davide Porrelli3, Gianluca Turco4
1Plastic and Reconstructive Surgery Unit, Cattinara Hospital, Strada di Fiume, 447, 34149 Trieste, Italy.
由原涂层,银纳米粒子功能化的聚烯酸纳米纤维制成的新型伤口敷料显示出出色的生物相容性和强大的抗菌特性. 这些先进的材料有效地支持细胞生长,同时抑制有害细菌,为更好的慢性伤口护理铺平了道路.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 纳米技术 纳米技术
背景情况:
- 慢性伤口需要具有细胞相容性和抗菌能力的先进生物材料.
- 电聚烯酸 (PCL) 纳米纤维膜为伤口愈合应用提供了一个有前途的支架.
- 用原和银纳米粒子 (AgNPs) 探索PCL的表面修饰,以提高其治疗潜力.
研究的目的:
- 为了评估原涂层和AgNP功能化的PCL纳米纤维膜的细胞相容性.
- 评估修饰PCL膜上的纤维细胞粘附,增殖和细胞毒性.
- 为了确定开发的伤口包装材料的抗微生物疗效.
主要方法:
- 在PCL,PCL-原,PCL-酸和PCL-原-AgNPs膜上培养了小鼠3T3纤维细胞.
- 细胞代谢活动使用Alamar Blue测定在24小时,48小时和72小时后得到量化.
- 细胞毒性通过乳酸脱酶 (LDH) 释放试验进行测量,并使用共聚焦显微镜可视化细胞活力/粘附. 对特定细菌菌株进行了抗菌活性测试.
主要成果:
- 所有测试的膜都支持纤维细胞生长,以原涂层的样本显示最高的代谢活性.
- 具有AgNP功能化的膜保持了90%以上的细胞活力,细胞毒性低 (约0. 10-20%) 的情况.
- 观察到完全抑制Pseudomonas aeruginosa和抗万科胺素的Enterococcus,以及部分抑制金黄色葡萄球菌.
结论:
- 原涂层,AgNP功能化的电PCL膜与纤维细胞具有显著的细胞相容性.
- 开发的材料对关键的伤口病原体具有强大的广泛抗菌活性.
- 这些发现支持这些改性PCL膜作为慢性伤口带的先进材料的潜力.
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