不针对性等离子体和脑脊液代谢物的纵向,个体内稳定性
Briana Rocha1, Erin M Jonaitis2,3, Alana Hamwi1
1Department of Population Health Sciences, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53726, USA.
Metabolites
|January 27, 2026
概括
本研究确定了可靠的血和脑脊液 (CSF) 代谢物,用于长期的流行病学研究. 我们描述了代谢物稳定性,尽管技术差异,但发现了适合纵向研究的特定子集.
科学领域:
- 代谢学 代谢学 代谢学
- 生物化学 生物化学
- 神经科学是一个神经科学.
背景情况:
- 纵向代谢学揭示了与衰老和健康相关的代谢途径的变化.
- 代谢物水平容易受到生物和技术变化的影响,影响数据可靠性.
- 确定稳定的代谢物对于长期的流行病学研究至关重要.
研究的目的:
- 评估非向性血和脑脊液 (CSF) 代谢产物的纵向和技术稳定性.
- 为了确定一组在长时间内保持可靠的代谢物的子集,用于流行病学研究.
- 了解代谢物稳定性如何在生物化学途径中变化.
主要方法:
- 利用了来自威斯康星州阿尔茨海默氏症预防注册 (WRAP) 和威斯康星州阿尔茨海默氏症研究中心 (ADRC) 的非定向超高性能液态染色体质谱学 (LC-MS) 代谢组数据.
- 分析了四个时间点的一般健康参与者的样本,跨越两个不同的分析浪潮 (2017年和2023年).
- 使用罗瑟里的类内相关系数 (ICCs) 量化了代谢物稳定性,并开发了一个综合得分,将纵向稳定性和技术方差整合起来.
主要成果:
- 在血和脑脊液中,代谢物的中位稳定性"相当" (罗瑟里 ρ > 0.40至≤0.75).
- 波间稳定性低于波内稳定性,这表明分析波之间的技术变异性增加.
- 已识别出具有"优异"稳定性和对批量效应的低敏感性的血和脑脊液代谢物子集,稳定性因生物化学超级通路而异.
结论:
- 突出特定的代谢物适合长期流行病学研究.
- 为实验设计和分析策略提供了洞察力,以结合不同队列和分析批次的数据.
- 强调在代谢学研究中考虑代谢物稳定性的重要性.
相关概念视频
01:21Cerebrospinal Fluid
5.7K
Cerebrospinal fluid (CSF) is a colorless liquid that flows around the brain and the spinal cord, playing a vital role in the protection, support, and overall function of the central nervous system (CNS). CSF production, circulation, and absorption are tightly regulated processes essential for the brain and spinal cord to function properly.
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain....
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain....
5.7K
02:20Longitudinal Research
13.2K
Sometimes we want to see how people change over time, as in studies of human development and lifespan. When we test the same group of individuals repeatedly over an extended period of time, we are conducting longitudinal research. Longitudinal research is a research design in which data-gathering is administered repeatedly over an extended period of time. For example, we may survey a group of individuals about their dietary habits at age 20, retest them a decade later at age 30, and then again...
13.2K
03:18Nuclear Stability
23.2K
Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...
To hold positively charged protons together...
23.2K
01:34The Fluid Mosaic Model
177.7K
The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.
177.7K
01:53RNA Stability
35.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.7K
01:26Longitudinal Studies
498
Longitudinal studies are also widely used in other medical and social science fields. For instance, in cardiovascular research, they can monitor patients' health over decades to identify risk factors for heart disease, such as high cholesterol or smoking, and evaluate the long-term effectiveness of preventive measures. Similarly, in mental health studies, researchers might follow individuals from adolescence into adulthood to understand the development and progression of conditions like...
498