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储存条件对脑脊液和血清中NSE,S100和B2M稳定性的影响
Natalia García-Sancha1, Juan Valencia2, Simón Gundín3
1Research Unit, Fundación Burgos por la Investigación de la Salud (FBIS), 16821 Hospital Universitario de Burgos , Burgos, Spain.
Clinical chemistry and laboratory medicine
|January 27, 2026
概括
大脑脊髓液 (CSF) 生物标志物神经元特异性酶 (NSE) 和S100在-20°C显著降解,影响诊断准确度. 在-80°C保存样品对于保持NSE和S100稳定性和确保可靠的生物标志物分析至关重要.
科学领域:
- 生物化学 生物化学
- 临床化学 临床化学
- 神经科学是一个神经科学.
背景情况:
- 对神经疾病的生物标志物的可靠性取决于样本的完整性.
- 大脑脊髓液 (CSF) 和血清储存条件显著影响生物标志物的稳定性.
- -80°C是黄金标准,但-20°C更容易获得,可能会造成蛋白质降解的风险.
研究的目的:
- 评估神经元特异性酶 (NSE),S100和-2微球蛋白 (B2M) 在CSF和血清中的稳定性.
- 为了比较 -20°C与 -80°C储存对这些生物标志物的影响,随着时间的推移.
- 确定最佳的储存条件,以便在神经诊断中进行可靠的生物标志物分析.
主要方法:
- 分析了来自儿科患者的63个脑液和56个血清样本.
- 在-20°C (24小时,6个月) 和-80°C (6,12个月) 储存的样本.
- 通过电化学发光测量NSE和S100;通过度免疫测试测量B2M.
主要成果:
- NSE和S100在-20°C显著不稳定,脑脊髓和血清显著下降.
- 在24小时内,CSF NSE在-20°C下降了~61%.
- 储存在-80°C保持NSE和S100稳定性 (在12个月内下降<15%);B2M在所有条件下保持稳定.
结论:
- 脑脊髓中的生物标志物NSE和S100在-20°C下高度易受降解.
- 在经过测试的储存条件下,β-2微球蛋白 (B2M) 显示稳定性.
- -80°C的储存对于保持NSE和S100的完整性至关重要,以便进行准确的诊断和研究.
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