在单细胞中对翻译后修改的的多重定量化,使用触发式MS/MS与超重双重质量标签相结合
Dong-Gi Mun1, Hiroshi Nishida1, Firdous A Bhat1
1Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905, United States.
Journal of proteome research
|January 27, 2026
概括
这项研究引入了一种新的向质谱法,用于对转化后修饰 (PTM) 的精确单细胞分析. 这种方法提高了在单个细胞中检测PTM的灵敏度和吞吐量.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 一个单细胞分析.
- 质谱测量质量谱测量
背景情况:
- 单细胞中无偏见的蛋白质组概况分析面临着缺少数据和低静态度转化后修饰 (PTM) 的可重复测量的挑战.
- 现有的方法在详细的单细胞PTM分析所需的灵敏度和吞吐量方面扎.
研究的目的:
- 制定一个有针对性的质谱策略,以在单细胞水平上对PTM进行敏感和可靠的量化.
- 克服缺少数据的局限性,提高单细胞PTM分析的可复制性.
主要方法:
- 开发了一个有针对性的策略,将双重质量标签 (TMT) 复杂化与基于SureQuant的触发MS / MS结合起来.
- 使用超重的TMT标记作为触发器来增强MS/MS检测.
- 建立了一种在单细胞分辨率下对基因素H3量化四个PTM的方法.
主要成果:
- 在单细胞中证明了素H3 PTMs (K14ac,K23ac,K27me,K27me3,K79me) 的强有力的定量.
- 与数据依赖采集和并行反应监测相比,显示了更好的灵敏度和吞吐量.
- 通过使用开发的策略,揭示了基因素PTM中的细胞异质性.
结论:
- 开发的有针对性的策略显著提高了单细胞PTM分析的灵敏度和吞吐量.
- 这种方法广泛适用于各种PTM,可以在单细胞中进行专注和精确的调查.
- 该方法提供了一个强大的工具,用于理解PTM中的细胞异质性.
更多相关视频
07:21Automated Sample Preparation for the Multiplexed Analysis of Single-Cell Histone Post-Translational Modifications (sc-hPTM2)
Published on: December 19, 2025
26.3K
09:06Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
Published on: May 1, 2017
7.4K
相关概念视频
Peptide Identification Using Tandem Mass Spectrometry
8.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.4K
Tandem Mass Spectrometry
2.4K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.4K
Post-translational Translocation of Proteins to the RER
7.7K
A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
7.7K
Translation
156.1K
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...
156.1K
Translation
17.8K
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
Translation Produces the Building Blocks of Life
Proteins are...
17.8K
Termination of Translation
27.6K
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
27.6K
