Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy03:07

Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy

29.8K
The kinetic molecular theory qualitatively explains the behaviors described by the various gas laws. The postulates of this theory may be applied in a more quantitative fashion to derive these individual laws.
29.8K
Range00:59

Range

14.1K
The range is one of the measures of variation. It can be defined as the difference between a dataset's highest and lowest values. For example, in the study of seven 16-ounce soda cans, the filled volume of soda was measured, thus producing the following amount (in ounces) of soda:
15.9; 16.1; 15.2; 14.8; 15.8; 15.9; 16.0; 15.5
Measurements of the amount of soda in a 16-ounce can vary since different subjects record these measurements or since the exact amount - 16 ounces of liquid, was not...
14.1K
Kinetic Energy00:23

Kinetic Energy

43.4K
Kinetic energy is the ability of an object in motion to do work or enact change. It can take on many forms. For instance, water flowing down a waterfall has kinetic energy. In biological systems, particles of light travel and are absorbed by plants to create chemical energy. Animals consume the chemical energy and give off molecules that carry their scent through the air. They also generate kinetic energy when they run away from predators. Entire systems also possess kinetic energy, like the...
43.4K
Enzyme Kinetics01:19

Enzyme Kinetics

104.0K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
104.0K
¹H NMR: Long-Range Coupling01:27

¹H NMR: Long-Range Coupling

2.7K
The coupling interactions of nuclei across four or more bonds are usually weak, with J values less than 1 Hz. While these are usually not observed in spectra, the presence of multiple bonds along the coupling pathway can result in observable long-range coupling.
In alkenes, spin information is communicated via σ–π overlap, as seen in allylic (four-bond) and homoallylic (five-bond) couplings. These coupling interactions are stronger when the σ bond is parallel to the alkene...
2.7K
Kinetic Friction01:26

Kinetic Friction

1.4K
Consider a truck trying to pull a stationary car. As the truck exerts a force on the car, static friction is created at the point of contact between the two surfaces. This frictional force resists the car's movement and keeps it at rest. However, when the applied force by the truck surpasses the limiting static frictional force, an interesting phenomenon occurs. The frictional force at the interface reduces to a lower value, known as the kinetic frictional force. At this point, the car...
1.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

CRISPR-Cas-Based Platform for Single-Step Quantification of Monoclonal Antibodies at Point-of-Care.

ACS sensors·2026
Same author

Reactive Hydrogen-Mediated Peroxydisulfate Activation for Boosting Carbon Nitride Electrochemiluminescence.

Analytical chemistry·2026
Same author

Microenvironment-engineered Brønsted acidity in MOF-808 for efficient organophosphate hydrolysis and sensing.

Biosensors & bioelectronics·2026
Same author

Metasurface-enhanced mid-infrared imaging spectroscopy with broadband quantum cascade lasers.

Nature communications·2026
Same author

Reversible bismuth reduction-driven dark photoelectrochemistry.

Nature communications·2026
Same author

Unconventional high-harmonic generation in resonant membrane metasurfaces.

Nature communications·2025

相关实验视频

Updated: Jan 29, 2026

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
11:00

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Published on: November 9, 2017

46.0K

一步式数字免疫试验中的动态分析能够在超广的动态范围中进行多重量化.

Abtin Saateh1, Rojina Allamehnejad1, Wenhong Yang1

  • 1Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.

Journal of the American Chemical Society
|January 27, 2026
PubMed
概括

这项研究引入了一种动力学框架,通过分析随时间推移的单粒子信号来克服免疫试验中的效应. 这种方法量化了分析物的高度,并减少了交叉反应性,以便在血清中准确检测生物标志物.

更多相关视频

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

28.9K
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

5.4K

相关实验视频

Last Updated: Jan 29, 2026

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
11:00

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Published on: November 9, 2017

46.0K
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

28.9K
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

5.4K

科学领域:

  • 生物感应是一种生物感应.
  • 分析化学 分析化学
  • 生物物理学的生物物理.

背景情况:

  • 一步三明治免疫试验在高分析剂度下可能会受到效应的影响,导致不准确的结果.
  • 效应发生在过多的分析物抑制三明治复合物的形成时,从而使定量分析复杂化.

研究的目的:

  • 开发一种动力学框架,以解决免疫试验中效应引起的模糊性.
  • 为了能够在广泛的动态范围内准确量化生物标志物,包括高度.
  • 在多重生物传感应用中解决和最大限度地减少交叉反应.

主要方法:

  • 从金纳米孔阵列中利用时间解析的单粒子等离子体信号与纳米粒子报道器.
  • 追踪了个体绑定事件,并使用大规模运输和反应有限模型分析了响应时间概况.
  • 开发了一个数字框架来分类和解多重测试中的交叉反应性.

主要成果:

  • 通过比较适应残留物,确定了对效应负责的动力过渡.
  • 通过了解底层动力学,将效应转化为定量特征.
  • 在未经加工的人类血清中成功应用了多重检测细胞因子和C反应蛋白的方法.
  • 能够同时量化超过9个数量级的生物标志物,而无需样本分割或稀释.

结论:

  • 建立了一个可概括的动力,交叉反应意识的生物感知范式.
  • 机械化策略克服了复杂生物样本传统免疫试验的局限性.
  • 这种方法提高了临床诊断中生物标志物量化的准确性和动态范围.