聚烯氧化酶在各种物理状态和度下在高压结下具有催化活性和一致性稳定性
Jinghao Li1, Yuanshan Yu2, Yujuan Xu1
1Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510640, China; College of Food Science and Technology, Zhongkai University of Agricultural and Engineering, Guangzhou 510631, China.
Food chemistry
|January 27, 2026
概括
高压结 (HPF) 影响了多氧化酶 (PPO) 的稳定性,液体PPO表现出更大的弹性. 通过改变PPO的物理状态来调节酶活性是可能的.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 食品科学 食品科学 食品科学
背景情况:
- 聚氧化酶 (PPO) 在食品色和质量方面至关重要.
- 了解PPO对高压冷 (HPF) 等物理处理的反应对于食品加工至关重要.
- PPO存在于不同的状态 (液体,固体) 和度,影响其性能.
研究的目的:
- 研究高压结 (HPF) 对多氧化酶 (PPO) 活性和结构的影响.
- 确定在HPF条件下影响PPO稳定性的关键因素.
- 探索通过物理状态操纵调节PPO活动的方法.
主要方法:
- 在不同压力 (0.1-400 MPa) 和温度 (-20°C) 处30分钟应用高压结 (HPF).
- 分析PPO酶活性和液体 (高/低度) 和固体晶体形式的结构变化.
- 使用反向传播的人工神经网络模型,将空间结构与酶活性相关联.
主要成果:
- 影响PPO活动和结构的顺序是:物理状态 > 度 > 压力 > 结晶路径.
- 在HPF下,液体PPO (LL-PPO) 与高度液体PPO (HL-PPO) 和固体晶体PPO (S-PPO) 相比表现出更高的稳定性.
- 低温对S-PPO的压力诱导变提供了保护作用.
- 硫含量和高度对于L-PPO的结构稳定性至关重要.
- 改变PPO的物理状态 (液体与固体) 通过激活和抑制有效调节酶活性.
- 一个反向传播的人工神经网络模型准确地预测了空间结构和酶活性之间的关系.
结论:
- 物理状态是HPF下的PPO稳定性的主要决定因素.
- HPF条件,特别是压力和温度,显著影响PPO结构和活动.
- 通过控制其物理状态,可以调节PPO活动,为食品质量管理提供潜力.
- 人工神经网络为了解酶结构-活性关系提供了一个强大的工具.
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