相关实验视频

Updated: Jan 29, 2026

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Published on: November 10, 2023

开发一种特定于链的RT-qPCR试验,用于检测和量化感染细胞中的博纳病病毒RNA

Takehiro Kanda¹, Shigeki Hattori², Ryo Komorizono³

¹Laboratory of RNA viruses, Department of Virus Research, Institute for Life and Medical Sciences, Kyoto University; Department of Molecular Virology, Graduate School of Medicine, Kyoto University.

Journal of virological methods

|January 27, 2026

概括

一种新的链特异性RT-qPCR试验区分了博纳病病毒1 (BoDV-1) RNA类型. 这种方法揭示了BoDV-1控制转录,而不是复制,对于持久性感染.

科学领域:

病毒学病毒学
分子生物学分子生物学
神经科学是一个神经科学.

背景情况:

博纳病病毒1 (BoDV-1) 是一种非细分的负链RNA病毒 (NSV),涉及致命的人类脑炎.
了解BoDV-1生命周期至关重要,因为它存在于哺乳动物的中枢神经系统中.
传统的RT-qPCR试验无法区分病毒RNA物种 (基因组,抗基因组,mRNA).

研究的目的:

开发一种新的链特异性RT-qPCR (ssRT-qPCR) 试验,用于区分BoDV-1RNA物种.
量化不同BoDV-1RNA分子的细胞内动力学.
阐明BoDV-1用于持续感染的机制.

主要方法:

使用修改后的RT-primer.开发一个特定链的RT-qPCR (ssRT-qPCR) 试验.
应用ssRT-qPCR测定方法来检测和量化BoDV-1的基因组,抗基因组和mRNA.
在感染细胞中测量细胞内RNA物种动力学.

主要成果:

通过ssRT-qPCR测定,成功地分化和量化了三种类型的BoDV-1RNAs.
细胞内动力学分析揭示了病毒RNA物种的独特模式.
研究结果表明BoDV-1调节转录,而不是复制,以维持持续的感染.

结论:

新的ssRT-qPCR试验是研究BoDV-1RNA动态的一个强大工具.
博德维-1的持续性可能与转录控制机制有关.
这种测试有助于更好地了解NSV生命周期和持久性感染.

关键词:

波尔纳病病毒1 波尔纳病病毒1 特定于一个链的RT-qPCR.持续的感染持续的感染.病毒转录和复制

更多相关视频

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

Published on: November 10, 2018

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

Published on: January 16, 2016

相关概念视频

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

Lagging Strand Synthesis

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...

Fixing Double-strand Breaks

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...