Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.3K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.3K
Constant Pressure Calorimetry03:02

Constant Pressure Calorimetry

97.6K
Calorimetry is a technique used to measure the amount of heat involved in a chemical or physical process or to measure the heat transferred to or from a substance. The heat is exchanged with a calibrated and insulated device called the calorimeter. Calorimetry experiments are based on the assumption that there is no heat exchange between the insulated calorimeter and the external environment. The well-insulated calorimeters prevent the transfer of heat between the calorimeter and its external...
97.6K
Enzymes02:34

Enzymes

94.6K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
94.6K
Constant Volume Calorimetry02:41

Constant Volume Calorimetry

30.7K
Calorimeters are useful to determine the heat released or absorbed by a chemical reaction. Coffee cup calorimeters are designed to operate at constant (atmospheric) pressure and are convenient to measure heat flow (or enthalpy change) accompanying processes that occur in solution at constant pressure. A different type of calorimeter that operates at constant volume, colloquially known as a bomb calorimeter, is used to measure the energy produced by reactions that yield large amounts of heat and...
30.7K
Calculating the Equilibrium Constant02:46

Calculating the Equilibrium Constant

37.9K
The equilibrium constant for a reaction is calculated from the equilibrium concentrations (or pressures) of its reactants and products. If these concentrations are known, the calculation simply involves their substitution into the Kc expression.
For example, gaseous nitrogen dioxide forms dinitrogen tetroxide according to this equation:
37.9K
Enzyme Kinetics01:19

Enzyme Kinetics

104.0K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
104.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

<i>In vivo</i> assessment of melanoma based on photoacoustic absorption and viscoelasticity imaging.

Biomedical optics express·2026
Same author

The effect of aircraft cockpit rudder pedal shape on lower limb muscle activation, plantar pressure, and comfort.

Applied ergonomics·2026
Same author

Discovery of High-Performance Extremophiles and Extremozymes Using Machine Learning and Structure-Based Clustering.

Environmental science & technology·2026
Same author

Synergistic polysulfide regulation and lithium deposition enabled by core-shell Ag/Ag<sub>2</sub>S heterostructured fabric.

Chemical communications (Cambridge, England)·2026
Same author

Probiotics: From natural to artificial.

Biotechnology advances·2026
Same author

Omega-based mini prime- and base-editing systems in Escherichia coli.

Trends in biotechnology·2026

相关实验视频

Updated: Jan 29, 2026

Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation
11:03

Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation

Published on: February 26, 2013

20.5K

使用iESCC准确的酶特异性常数预测.

Yu Zhang1, Li-Hua Liu2, Shuqi Wang2

  • 1Biosciences Central Research Facility, The Hong Kong University of Science and Technology, GuangZhou 510000, China.

Bioresource technology
|January 27, 2026
PubMed
概括

一个新的深度学习模型,iESC,从酶序列和基质结构准确地预测了诸如迈凯利斯常数 (Km) 和循环数 (kcat) 等酶动态参数,加速了酶研究.

关键词:
深度学习是一种深度学习.深度突变扫描 进行深度突变扫描.酶特异性常数 酶特异性常数功能提取 功能提取高通量选的高通量选

更多相关视频

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
06:04

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae

Published on: February 24, 2023

2.9K
A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds
13:34

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

10.6K

相关实验视频

Last Updated: Jan 29, 2026

Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation
11:03

Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation

Published on: February 26, 2013

20.5K
Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
06:04

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae

Published on: February 24, 2023

2.9K
A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds
13:34

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

10.6K

科学领域:

  • 生物化学 生物化学
  • 计算生物学 计算生物学
  • 酶动力学 酶动力学

背景情况:

  • 酶特异性常数 (ESC),包括迈凯利斯常数 (Km) 和周转数 (kcat),对于理解酶功能至关重要.
  • 确定Km和kcat的传统方法耗费大量资源和时间,阻碍了快速的酶表征.

研究的目的:

  • 开发和验证iESC,一种新的深度学习模型,只使用酶序列和基质结构来准确预测酶运动参数 (Km,kcat和kcat/Km).
  • 为了证明iESC的优越性能与现有的最先进的模型相比.

主要方法:

  • 在iESC的训练中,使用了41,907个酶基质动态参数的大数据集,这些数据集经过了精心策划和预处理,以获得准确性和独立性.
  • 该模型将高级特征提取技术与深度学习架构集成在一起.
  • 使用确定系数 (R2),根平均平方误差 (RMSE) 和平均绝对误差 (MAE) 评估性能,并与其他模型进行基准测试.

主要成果:

  • iESC实现了高预测精度,Km的R2值为0.63,kcat为0.60,kcat/Km为0.62.
  • 基准测试证实iESC的显著优于现有模型的性能,显示出更高的R2和更低的RMSE和MAE.
  • 该模型在高通量查 (HTS) 和深度突变扫描 (DMS) 环境中显示出强大的适用性.

结论:

  • iESC提供了一种快速,准确和基于序列的方法来预测关键的酶动力学参数.
  • 该模型对加速酶发现,工程和功能表征在各种生物技术应用中具有重大意义.