周围血液单核细胞线粒体生物能学与年轻和老年人的血管内皮功能有关
S Tony Wolf1, James F Bangle1, William E Jennings1
1Department of Kinesiology, University of Georgia, Athens, GA, 30602.
概括
衰老会损害外围血液单核细胞 (PBMC) 中的线粒体功能,导致抗氧化能力降低和内皮功能障碍. 这些发现突出了细胞生物能学的关键与年龄相关的变化,影响了血管健康.
科学领域:
- 心血管研究研究心血管研究
- 线粒体的生物能量学
- 生物老龄化生物学
背景情况:
- 周围血液单核细胞 (PBMC) 越来越多地被用于心血管研究.
- 对于PBMC线粒体生物能学和氧化应激的与年龄相关的变化,人们的理解有限.
- 这些细胞变化与内皮功能之间的关系在很大程度上仍未被探索.
研究的目的:
- 在PBMC中调查线粒体生物能学和抗氧化剂缓冲能力 (AoxBC) 中与年龄相关的差异.
- 确定这些PBMC细胞变化和内皮功能 (流介导扩张,口腔疾病) 之间的关联.
主要方法:
- 在年轻和老年人中使用高分辨率呼吸计和度计评估线粒体呼吸率 (JO2) 和膜潜力 (Δψm).
- 测量了线粒体反应性氧物种 (mtROS) 排放和产生,计算了AoxBC.
- 通过流媒扩张 (FMD) 量化内皮功能.
主要成果:
- 与年轻人相比,老年人在更高的能量状态下表现出较低的线粒体呼吸和低极化线粒体膜潜力 (Δψm).
- 抗氧化剂缓冲能力 (AoxBC) 在老年人中显著降低.
- 与年龄相关的JO2和Δψm的下降与口炎受损相关,表明内皮功能受损.
结论:
- 随着年龄的增长,PBMC线粒体的生物能量会发生变化,其特点是呼吸能力和膜潜力下降.
- 在老年人中AoxBC的降低表明细胞对氧化应激的防御能力下降.
- 这些PBMC线粒体变化与与年龄相关的血管内皮功能障碍有关.
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