开发用于生产真菌天然产品的微生物底盘
Junyang Wang1,2, Xu Ji1, Zhenlin Xin1
1Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Green Biomanufacturing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Critical reviews in biotechnology
|January 27, 2026
概括
天然产品 (FNP) 具有药用潜力,但很难生产. 本综述探讨了利用合成生物学和异质表达来解锁沉默的真菌生物合成基因集群 (BGCs),以有效地发现和生产FNP.
科学领域:
- 生物化学 生物化学
- 合成生物学 合成生物学
- 自然产品 化学 化学
背景情况:
- 真菌天然产品 (FNP) 是重要的药物,许多潜在化合物存在于静默的生物合成基因集群 (BGC).
- 在本地真菌宿主中激活这些沉默的BGCs是具有挑战性的,因为遗传操纵困难和低代谢产量.
- 由合成生物学实现的异质表达系统提供了一个有希望的替代方案来克服这些局限性.
研究的目的:
- 审查FNP及其生物合成酶的结构类别.
- 评估微生物底盘和通路重构策略,以有效地表达FNP的异质表达.
- 讨论优化方法,以提高FNP的生产和最大限度地减少异质系统的副产品.
主要方法:
- 关于FNP结构类和骨干酶的文献综述.
- 评估微生物底盘适合异质表达的情况.
- 对FNP生物合成路径重构和优化策略的分析.
- 讨论真菌天然产品异质生产中的挑战和未来方向.
主要成果:
- 确定主要的FNP结构类和相关酶.
- 对各种微生物宿主和合成生物学工具进行评估,用于异种FNP生产.
- 编制路径工程策略,以提高FNP标位和纯度.
- 对目前的局限性进行分析,并提出改善异质FNP生物合成的解决方案.
结论:
- 异质表达系统对于释放沉默的真菌BGCs的潜力至关重要.
- 有效的路径重构和优化是有效的FNP发现和生产的关键.
- 微生物底盘和合成生物学工具的持续开发将推动FNP生物合成.
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