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在肠道微生物群中的细菌基因组DNA的大规模囊介导动员
Tatiana Borodovich1, Colin Buttimer1, Jason S Wilson2
1APC Microbiome Ireland & School of Microbiology, University College Cork, Cork, Ireland.
Nature communications
|January 27, 2026
概括
细菌DNA被病毒类囊包装在肠道微生物群中很常见. 纳米孔测序揭示了广泛的基因转移剂 (GTA) 和菌体活动,特别是在奥斯科科科科和鲁米诺科科科科家族中.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 基因组学就是基因组学.
背景情况:
- 菌体和基因转移剂 (GTA) 在结构性囊体的限制内包装宿主DNA.
- 了解肠道微生物群中的细菌DNA包装的范围和机制对于微生物生态学至关重要.
研究的目的:
- 精确识别包装在人类肠道微生物群中的病毒囊中的细菌DNA.
- 研究细菌DNA包装机制的流行和多样性,包括通用转导,GTA活性和prophage诱导.
主要方法:
- 利用完整的纳米孔测序,囊包装的DNA分子,以获得全长的读取.
- 将该方法应用于健康捐赠者的便病毒组,以分析封装DNA.
- 通过使用具有良好的特征的传感系统验证了方法.
主要成果:
- 细菌DNA包装在肠道微生物群中普遍存在,高达5.4%的囊包装DNA来源于细菌基因组.
- 一般转导和GTA活性在奥斯基洛斯皮拉属和鲁米诺可可属中突出 (例如,Faecalibacterium spp. ) 的情况.
- 在 Bacteroides 中观察到侧向转导,在普遍存在的肠道细菌种群中检测到 prophage 诱导.
结论:
- 病毒或类似病毒的囊在人类肠道中广泛包装细菌DNA.
- 这种包装有助于水平基因转移,并塑造肠道微生物社区结构.
- 纳米孔测序为研究囊包装DNA和微生物基因转移提供了强大的工具.
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