使用LC-MS/MS和体外功能测定方法对Hypericum perforatum提取物进行综合化学和生物表征
Mehmet Ali Güzel1, Turgay Kolaç2, İrem Nur Menevşe3
1Health Services Vocational School, Inonu University, Malatya, 44280, Turkey.
Scientific reports
|January 27, 2026
概括
野生圣约翰草 (Hypericum perforatum) 提取物显示出强大的抗氧化和抗癌特性. 这项研究确定了新型化合物,并证实了显著的生物活性,包括在癌细胞中诱导亡.
科学领域:
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
- 药用植物 药用植物
背景情况:
- (圣约翰草) 以其丰富的二次代谢物和治疗潜力而闻名.
- 野生种群提供了多样化的植物化学特征的来源.
- 了解植物化学成分对于解锁其药理学应用至关重要.
研究的目的:
- 综合分析野生H. perforatum甲醇提取物的植物化学成分.
- 评估这些提取物的抗氧化,抗癌和抗微生物生物活性.
- 为了在植物的概况中识别新的化合物.
主要方法:
- 野生H. perforatum的甲醇提取.
- 液体染色学-并联质谱学 (LC-MS/MS) 用于化合物识别.
- 在体外抗氧化剂测试 (DPPH,ABTS),针对癌细胞系的细胞毒性测试,用于细胞亡和细胞周期分析的流动细胞计和抗菌测试.
主要成果:
- 在LC-MS/MS中发现了36种化合物,其中包括4种新化合物 (genkwanin,vicenin-2,schaftoside,afzelin).
- 提取物显示出显著的抗氧化活性 (含量:203.04毫克GAE/g DW;DPPH:544.78毫克TE/g DW;ABTS:312.15毫克TE/g DW).
- 在体外对癌细胞有显著的细胞毒性 (IC50在A549中低至3.57μg/mL),诱导细胞亡和G0/G1细胞周期停止. 观察到对大肠杆菌和金黄色杆菌的适度抗菌活性.
结论:
- 野生H. perforatum是一种有价值的生物活性化合物来源,具有显著的抗氧化和抗癌特性.
- 已识别的化合物,包括新的化合物,有助于植物的治疗潜力.
- 需要进一步的研究来探索H. perforatum提取物及其成分的临床应用.
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