在血和口服液中通过LC-MS/MS检测和量化阿片类药物
Luana M Rosendo1,2, Suzel Costa3, Susana Simões3
1RISE-Health, Departamento de Ciências Médicas, Faculdade de Ciências da Saúde Universidade da Beira Interior (FCS-UBI), Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
Analytical and bioanalytical chemistry
|January 27, 2026
概括
一种新的液体色谱-并联质谱法 (LC-MS/MS) 方法可以准确地检测血和口服液中的多种阿片类药物. 这种敏感的测试支持对正在进行的阿片类药物危机进行非侵入性监测.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 阿片类药物危机需要先进的分析方法来检测毒品.
- 在生物矩阵中精确量化阿片类药物对于患者监测和法医调查至关重要.
研究的目的:
- 开发和验证一种敏感的液体染色学-并联质谱法 (LC-MS/MS) 方法.
- 在血和口服液中同时检测和量化六种关键阿片类药物 (芬太尼,布普伦诺芬,氧化,吗啡,特拉马多尔,塔潘塔多尔).
主要方法:
- 方法验证遵循FDA的指导方针,评估选择性,线性,精度,准确性,矩阵效应,提取效率,稳定性,转移和稀释完整性.
- 液体染色学-双重质谱法 (LC-MS/MS) 用于敏感的检测和量化.
- 对真实血和口服液样本进行了分析.
主要成果:
- 经过验证的LC-MS/MS方法表现出高灵敏度与低量化极限 (LLOQs),从0.1 ng/mL开始用于芬太尼.
- 实现了极好的精度和准确性,变化系数低于15%.
- 大多数分析品的提取效率超过了90%,矩阵效应处于可接受范围之内. 口服液被证明是可行的阿片类药物检测矩阵.
结论:
- 开发的LC-MS/MS方法强大,可靠,适合在血和口服液中同时检测阿片类药物.
- 口服液作为一个有前途的非侵入性替代或补充的矩阵用于阿片类药物监测血.
- 这种方法对法医毒理学和临床药物监测在管理阿片类药物危机方面具有重要意义.
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