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一个复杂的互换转位与松散的葡萄藤体变异体的雌同体活力降低有关
Noelia Alañón-Sánchez1, Yolanda Ferradás1,2, Ilja Bezrukov3
1Instituto de Ciencias de la Vid y del Vino , ICVV; CSIC, Gobierno de La Rioja, Universidad de La Rioja, Logroño, 26007 , Spain.
一种葡萄树体变体 (VP11) 带有较宽松的葡萄束,由于相互转位 (Tra1-3),其果实数量减少. 这种遗传缺陷降低了雌同体的生存能力,在植物繁殖作物中提供了好处.
科学领域:
- 植物遗传学和育种.
- 基因组学就是基因组学.
- 葡萄种植 葡萄种植 葡萄种植
背景情况:
- 葡萄藤 (Vitis vinifera L.) 品种由于高异性而需要克隆繁殖.
- 身体突变可以产生有益的特征,例如紧品种的松散束,改善成熟和减少腐烂.
- VP11是一种松散的体质变体"Tempranillo Tinto"与减少的水果设置.
研究的目的:
- 调查葡萄酒变体VP11.1中松特征的遗传基础.
- 了解结构变体和减少水果群之间的关系.
主要方法:
- 对VP11的表型和遗传分析.
- 长读全基因组测序以识别体质结构变异 (SV).
- 确定了SV的分子验证和后代遗传的分析.
主要成果:
- 与对照组相比,VP11的花粉活力和种子数量减少了50%左右.
- 在VP11中确定了11个大型SV,包括互换转位 (Tra1-3) 和细分重复.
- 特拉1-3与后代的花粉活力显著降低有关,不平衡的配体是不可活性的.
结论:
- 一个异构的互换转位 (Tra1-3) 与松葡萄变体VP11.11中的雌同体活力降低有关.
- 减少的种子和水果,从而带来农学益处,可能会导致植物繁殖作物的遗传缺陷的选择.
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