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相关概念视频

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Sequences01:29

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Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Arithmetic Sequences01:30

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An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...
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Next-generation Sequencing03:00

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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相关实验视频

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3' End Sequencing Library Preparation with A-seq2
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SCITO-seq2:超高通量单细胞转录组和表位组测序

Su-Hyeon Lee1, Bo-Yeong Jin2,3, Cho-Rong Lee4

  • 1Department of Biomedical Sciences, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.

Genome biology
|January 28, 2026
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此摘要是机器生成的。

在超过10万个细胞中,SCITO-seq2通过结合RNA和蛋白质分析来推进单细胞多组学. 这种具有成本效益的平台通过识别免疫细胞特征来增强自身免疫疾病研究.

关键词:
具有自身免疫透的CTLA4哈普洛缺陷.儿童系统性红血性狼单细胞多组体的单细胞多组体超高通量测序 超高通量测序

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科学领域:

  • 免疫学 免疫学 免疫学
  • 基因组学就是基因组学.
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 单细胞多组技术对于理解复杂的生物系统至关重要.
  • 现有的方法在可扩展性,成本效益和精确的分子形状匹配方面面临挑战.

研究的目的:

  • 推出SCITO-seq2,这是一个改进的超高通量单细胞RNA和蛋白质分析平台.
  • 为了证明SCITO-seq2在剖析自身免疫性疾病中免疫细胞异质性的实用性.

主要方法:

  • SCITO-seq2集成了基于探针的RNA检测与使用共享池条形码的蛋白质分析.
  • 该平台支持细胞散列,以实现高效的样本复杂化.
  • 对来自自身免疫性疾病队列的10万多个细胞进行了分析.

主要成果:

  • SCITO-seq2在一个大型细胞群中实现了对转录和表面蛋白质的可靠量化.
  • 通过条形码策略,可以确保多重滴中的分子形状精确匹配.
  • 该平台成功识别了自身免疫性疾病中的小免疫群和疾病特异性蛋白质特征.

结论:

  • SCITO-seq2提供了一个可扩展,精简和具有成本效益的下一代单细胞多omics工作流.
  • 这项技术可以更深入地了解自身免疫性疾病背后的细胞机制.
  • SCITO-seq2有助于发现新的生物标志物和治疗点.