轻度臭氧诱导的氧化应激调节了猪中性粒细胞和单细胞的活性和活力
Dominika Nguyen Ngoc1, Jose Luis Valverde Piedra2, Andrzej Milczak3
1Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland.
Animals : an open access journal from MDPI
|January 28, 2026
概括
臭氧治疗是一种臭氧疗法.
科学领域:
- 免疫学 免疫学 免疫学
- 生物医学科学 生物医学科学
- 毒理学 毒理学 毒理学
背景情况:
- 臭氧 (O3) 是一种具有越来越多生物医学应用的活性氧化剂.
- 它对先天性免疫细胞的剂量依赖性影响,特别是在非人类物种中,需要进一步定义.
- 了解这些影响对于安全的治疗用途至关重要.
研究的目的:
- 研究两种臭氧 (O3) 暴露方案 (30微克/毫升和90微克/毫升) 对猪中性粒细胞和单细胞的影响.
- 评估细胞活力,反应性氧和物种 (RONS) 生产以及暴露后的关键酶活动.
- 阐明中性粒细胞和单细胞对臭氧的不同反应.
主要方法:
- 猪外周血液中性粒细胞和单细胞被分离出来.
- 细胞暴露于30微克/毫升和90微克/毫升的臭氧 (O3).
- 细胞活力,RONS产生以及髓氧化酶,弹性酶,性酸酶和酸酶活性在1小时和24小时后被测量.
主要成果:
- 较低的臭氧 (O3) 剂量 (30μg/mL) 引起了轻微的免疫细胞激活,但没有影响生命力.
- 高剂量 (90μg/mL) 诱导了显著的氧化应激,增强了脱粒化,并将中性粒细胞活力降低了60%以上.
- 中性粒细胞表现出比单细胞更明显的反应,在较低剂量下保持活力,但在较高剂量下表现出功能受损.
结论:
- 臭氧 (O3) 具有双重作用:可控剂量可以调节免疫力,而过量剂量会损害细胞功能.
- 猪中性粒细胞比单细胞对臭氧更敏感.
- 定义臭氧 (O3) 应用的安全有效的治疗窗口是必不可少的.
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