使用一种体外发酵模型来评估Eimeria-扰乱的Cecal微生物组和代谢组上的益生菌
Yani Wu1,2, Xueting You3, Shuping Huang1
1Key Laboratory of Qinghai Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Southwest Minzu University, Chengdu 610041, China.
Animals : an open access journal from MDPI
|January 28, 2026
概括
在Eimeria tenella感染后,用Lactobacillus rhamnosus (LR) 或Bacillus subtilis (BS) 益生菌修复 brojler cecal微生物组显示出明显的效果. LR益生菌直接调节了异生菌微生物群中的关键代谢干扰.
科学领域:
- 微生物学 微生物学
- 动物科学动物科学
- 胃肠病学 胃肠病学
背景情况:
- 艾梅里亚感染会扰乱 brojler cecal 微生物组,影响生长.
- 了解宿主独立的益生菌作用对于缓解菌病至关重要.
研究的目的:
- 在试验室中研究乳杆菌 (Lactobacillus rhamnosus,LR) 和细菌 (Bacillus subtilis,BS) 对被埃米里亚菌扰乱的细胞微生物组和代谢组的直接影响.
- 为了比较LR和BS在恢复微生物和代谢平衡方面的有效性.
主要方法:
- 一个使用 brojler cecal slurry 的体外发酵模型.
- 治疗包括健康对照,感染Eimeria的对照,LR补充和BS补充组.
- 进行了16S rRNA测序和非目标代谢分析.
主要成果:
- 埃梅里亚感染增加了微生物多样性,促进了病原体 (Helicobacter, Bacteroides) 和抑制了共生菌 (Lactobacillus),改变了530种代谢物.
- 补充LR恢复了107种代谢物,影响了银河糖代谢和膜脂平衡.
- 补充BS恢复了64种代谢物,主要与二次代谢有关.
结论:
- 拉科巴西勒斯 (Lactobacillus rhamnosus) 直接调节埃梅里亚诱导的异生菌微生物群中的关键代谢干扰.
- 细菌的作用可能更多地依赖于宿主介导的相互作用.
- 益生菌显示出纠正因Eimeria引起的肠道失生症的潜力.
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