DOG1 免疫组合化学表达在正常和新发性狗组织中:它只是GIST的标记物吗?
Maria Morini1, Francesca Gobbo1, Luciana Mandrioli1
1Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
Animals : an open access journal from MDPI
|January 28, 2026
概括
狗的DOG1蛋白在各种组织和瘤中表达,而不仅仅是GIST. 将DOG1和KIT标记物结合起来,可以改善对狗胃肠道瘤 (GISTs) 的诊断.
科学领域:
- 兽医病理学 兽医病理学
- 在瘤学瘤学.
- 免疫组织化学 免疫组织化学
背景情况:
- 在人类的GIST中,DOG1蛋白具有很高的表达.
- 狗的DOG1表达是有限的,诊断价值与GISTs的KIT相美.
- 在其他狗组织中DOG1的表达在很大程度上没有特征.
研究的目的:
- 评估DOG1在正常和瘤犬组织中的表达.
- 评估DOG1在大型犬类GIST群体中的诊断作用.
- 将DOG1表达与狗GIST中的KIT进行比较.
主要方法:
- 对143个FFPE狗样本 (55个正常,88个瘤) 的免疫组织化学分析.
- 评估DOG1表达模式和强度.
- 在狗GIST中对DOG1和KIT表达的比较.
主要成果:
- DOG1在几种犬类细胞类型中表达强烈,部分反映了人类的模式.
- 在狗的GIST中,DOG1的灵敏度为92% (KIT:96%).
- 一个KIT阴性/DOG1阳性瘤突出了结合标记物的必要性.
- 在其他犬类瘤中观察到中度至强度的DOG1表达.
结论:
- DOG1在GISTs以外的各种犬类组织和瘤中表达.
- 将DOG1和KIT结合起来,可以提高犬类GIST的诊断准确度.
- 需要进一步研究DOG1在犬类恶性瘤中的作用.
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