微囊素-LR诱导的氧化应激,转录组变化,肠道微生物群,以及Rana chensinensis的组织病理学
You Wang1, Bingjie Wang1, Zhuolin He1
1Department of Ecology, College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
Animals : an open access journal from MDPI
|January 28, 2026
概括
微囊素-LR (MC-LR) 暴露会损害中国棕色青的,导致氧化应激,基因表达的改变和肠道微生物群的变化. 观察到组织学损伤,突出显示了MC-LR.
科学领域:
- 环境毒理学环境毒理学
- 水生生态毒理学 水生生态毒理学
- 两动物毒理学
背景情况:
- 菌产生微囊素-LR (MC-LR),这种毒素存在于各种水体中,对水生生物构成威胁.
- 暴露于MC-LR的anuran人的综合毒理终点,包括氧化应激,基因表达,肠道微生物群和组织病理学,尚未得到充分理解.
研究的目的:
- 为了研究MC-LR对氧化应激,转录组,肠道微生物群和中国棕色青的基因病理学的影响.
- 为了评估MC-LR在子中的剂量依赖性毒性.
主要方法:
- 幼暴露于MC-LR的度为0,0.1,1.0,5.0和10.0μg/L,持续了7天.
- 氧化应激标志物 (MDA,SOD,GPx,TAC),肝脏基因表达 (RNA-seq),肠道微生物群组成 (16S rRNA测序) 和组织病理学都被分析.
- 进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
主要成果:
- 高的MC-LR剂量 (10.0μg/L) 显著增加了MDA并降低了SOD,GPx和TAC.
- 暴露于MC-LR改变了基因表达,在1.0μg/L时达到2361 DEG,在10.0μg/L时达到3185 DEG.
- MC-LR影响了与消化相关的途径,改变了细菌的丰富性和组成,并导致肝脏和肠道的组织学损伤.
结论:
- MC-LR 诱导氧化应激,影响基因表达,破坏肠道微生物群,并导致青幼的组织病理损伤.
- 这些发现提供了对anurans中MC-LR毒性的机制的见解.
- 这项研究强调了新鲜水生态系统中MC-LR污染的生态风险.
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