在皮肤状细胞癌中快速评估瘤厚度,使用ex vivo聚焦显微镜
Daniela Hartmann1,2, Katharina Wex1, Aimée Braun1
1Department of Dermatology and Allergy, LMU University Hospital, Ludwig Maximilian University of Munich, 80337 Munich, Germany.
Cancers
|January 28, 2026
概括
活体共聚焦激光扫描显微镜 (EVCM) 准确地测量皮肤状细胞癌 (cSCC) 瘤厚度. 这种快速的内科手术工具有助于评估转移风险,并在手术期间对瘤进行分类.
科学领域:
- 皮肤病学 皮肤病学
- 在瘤学瘤学.
- 医疗成像医学成像
背景情况:
- 活体共聚焦激光扫描显微镜 (EVCM) 提供了手术期间新鲜组织的快速,手术内分析.
- 它作为一种有价值的辅助常规组织学非黑色素细胞皮肤癌 (NMSC) 评估.
- 以前的研究证实了EVCM识别cSCC形态特征的能力,但定量准确性,如瘤厚度测量,仍然未被评估.
研究的目的:
- 评估EVCM在测量皮肤状细胞癌 (cSCC) 瘤厚度中的准确性和可重复性.
- 确定EVCM是否可靠地根据瘤厚度对转移风险评估对cSCC进行分类.
主要方法:
- 八十二个组织学验证的cSCC样本使用EVCM和组织病理学 (黄金标准) 进行了分析.
- 对照性瘤厚度 (CTT) 和组织病理性瘤厚度 (HTT) 在统计上进行了比较.
- 评估了EVCM在分配cSCCs以纠正瘤厚度类别中的可靠性.
主要成果:
- 在EVCM和组织病理学之间观察到很高的一致性,Spearman相关系数为0.94,R平方为0.859.
- EVCM正确地将95.1%的cSCC分类到各自的瘤厚度类别.
- 科恩的卡帕值为0.90,表明这两种方法之间几乎完全一致.
结论:
- EVCM是一种精确可靠的方法来确定cSCC瘤厚度及其分类.
- 该技术允许立即在手术期间评估转移风险,并对低风险瘤进行初步分类.
- 建议在更大的队列中进一步验证临床实施.
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