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Updated: Jan 29, 2026

Analysis of Gene Expression in Emerald Ash Borer Agrilus planipennis Using Quantitative Real Time-PCR
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在2D和3DAML12肝细胞模型中进行定量PCR分析的参考基因验证

Zhenya Ivanova1, Valeria Petrova1, Betina Todorova1

  • 1Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria.

Biomedicines
|January 28, 2026
PubMed
概括

在比较2D和3D细胞培养时,选择稳定的家政基因进行基因表达分析至关重要. 这项研究确定了Hprt,Ppia和Actb作为这些模型中准确RT-qPCR正常化最可靠的参考基因.

关键词:
在2D/3D细胞培养中.在AML12细胞中,进行RT-qPCR规范化.在体外肝细胞模型.基因基因基因基因基因基因基因基因基因基因

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