光生物调节调节了莱德龙酸处理的骨质细胞类SaOs-2细胞的反应:对与双酸盐相关的骨髓缩的影响
Rodrigo Antonio Carvalho Andraus1, Ana Flávia Spadaccini Silva de Oliveira2, Mário Celso Teixeira Lopes3
1Graduate Program in Human Movement and Rehabilitation, Evangelical University of Goias (UniEVANGÉLICA), Anápolis 75083-515, GO, Brazil.
Bioengineering (Basel, Switzerland)
|January 28, 2026
概括
激光光生物调制 (PBM) 疗法可以防止龙酸 (ZA) 的副作用. 808nm的PBM与1J刺激了细胞活性,并在骨髓瘤细胞中增加了保护性BCL-2基因表达.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 在瘤学瘤学.
背景情况:
- 与双酸相关的骨死 (BRONJ) 是酸治疗 (ZA) 的严重副作用.
- 骨肉瘤细胞 (SaOs-2) 用于在体外模型 BRONJ.
- 激光光生物调制 (PBM) 正在研究治疗应用.
研究的目的:
- 评估PBM对用ZA治疗的SaOs-2细胞的影响.
- 为了确定PBM是否可以减轻ZA诱导的细胞毒性.
- 分析PBM对细胞代谢活动,细胞亡和基因表达 (BAX和BCL-2) 的影响.
主要方法:
- 在24小时内,SaOs-2细胞接受了100μM ZA的治疗.
- 随后,细胞在波长为660nm和808nm的波长下暴露于PBM,能量剂量各不相同 (1,5,10,20 J).
- 在PBM治疗后24小时评估了代谢活性,亡,BAX和BCL-2基因表达.
主要成果:
- ZA显著降低了SaOs-2细胞中的代谢活性.
- 808nm的PBM与1J减弱的ZA诱导的代谢活动减少.
- PBM以波长和剂量依赖的方式调节BCL-2基因表达,在808nm (1J和20J) 显著上调.
- PBM没有逆转ZA诱导的亡.
结论:
- PBM疗法对用ZA治疗的SaOs-2细胞表现出波长和剂量依赖的影响.
- 在808nm和1J的PBM显示出刺激细胞代谢活动和升调BCL-2表达的潜力.
- 这些发现表明,在BRONJ模型中,PBM对ZA诱导的细胞毒性具有潜在的保护作用.
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