装有bFGF的PDA微粒增强工程皮肤的血管化,同时增加白细胞的招募
Britani N Blackstone1, Zachary W Everett1, Syed B Alvi2,3
1Department of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.
Bioengineering (Basel, Switzerland)
|January 28, 2026
概括
装有基本纤维细胞生长因子 (bFGF) 的聚多巴胺 (PDA) 微粒改善了小鼠的工程皮肤 (ES) 血管化. 然而,需要最佳的bFGF度来平衡血管生长与减少炎症和健康的皮肤结构.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 工程皮肤 (ES) 是对大伤的有希望的治疗方法.
- 延迟血管化是ES移植失败的主要原因之一.
- 聚多巴胺 (PDA) 微粒为控制生长因子的输送提供了一个潜在的解决方案.
研究的目的:
- 评估载有基本纤维细胞生长因子 (bFGF) 的PDA微粒在加速ES血管化的有效性.
- 为了评估bFGF载荷的PDA微粒对ES结构和体内炎症反应的影响.
主要方法:
- 人体纤维细胞和角质细胞被用来在原基架上创建皮肤模板 (DTs) 和ES.
- 带有不同bFGF度的PDA微粒被纳入了脚手架.
- DTs和ES在试验室和在免疫缺陷小鼠的全厚伤口上移植后进行了评估.
主要成果:
- 在体外,bFGF载荷的PDA微粒增强了DT代谢活性,但这种效应在ES中减弱了.
- 在体内,bFGF载荷的PDA微粒显著增加了血管形成 (CD31+),以剂量依赖的方式.
- 带有bFGF载荷的PDA微粒的ES显示水分流失增加,表皮结构发生变化,炎症细胞透增加.
结论:
- PDA微粒是ES中生长因子的可行传递系统.
- 进一步优化bFGF度或探索替代生长因子是必要的,以促进血管化,同时减轻对表皮完整性和炎症的不良影响.
相关概念视频
01:18Increasing Function
389
An increasing function exhibits a rise in output values as input values increase. This behavior is depicted graphically as a curve or line that slopes upward from left to right. Such a function satisfies the condition that if x1 < x2, then f(x1) < f(x2), indicating that the function values grow with increasing inputs. This concept is fundamental in understanding growth trends across various domains, such as population dynamics, financial investments, or resource consumption.The...
389
01:27Disorders of Leukocytes
1.9K
Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune...
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune...
1.9K
01:30Classification of Leukocytes
5.7K
Leukocytes are classified into two groups based on the presence or absence of cytoplasmic granules. Granular leukocytes, which contain granules, belong to the myeloid lineage and are divided into three subtypes: neutrophils, eosinophils, and basophils. These cells are roughly spherical and characterized by the granules in their cytoplasm.
Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...
Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...
5.7K
03:24Seedless Vascular Plants
66.8K
Seedless Vascular Plants Were the First Tall Plants on Earth
66.8K
00:49What is Genetic Engineering?
80.0K
Overview
80.0K
03:00Self-Evaluation: Self-Enhancement and Self-Verification
5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K