多花蜂蜜对干细胞衰老的保护作用在动态细胞培养模型中
Fikriye Fulya Kavak1,2, Sara Cruciani1, Giuseppe Garroni1
1Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.
Antioxidants (Basel, Switzerland)
|January 28, 2026
概括
蜂蜜预处理通过调节参与多能性和衰老的关键基因,保护皮肤干细胞免受紫外线衰老的影响. 它还会影响Wnt信号通路,这表明它是一种天然的方法来对抗皮肤衰老.
科学领域:
- 皮肤病学和再生医学
- 自然化合物的生物化学.
- 细胞生物学和衰老问题
背景情况:
- 天然化合物,如蜂蜜类黄和化合物,在缓解皮肤衰老和氧化应激方面表现有前途.
- 紫外线 (UV) 辐射诱导皮肤衰老和细胞损伤,影响干细胞功能.
- 与无翼细胞相关的整合部位 (Wnt) 信号通路在干细胞调节和分化中起着至关重要的作用.
研究的目的:
- 研究蜂蜜预处理对皮肤干细胞 (SSC) 和人类皮肤纤维细胞 (HFF1) 对抗紫外线诱导的衰老的保护作用.
- 评估蜂蜜对干细胞相关基因,多能性标记物,衰老调节物和Wnt信号通路的影响.
- 评估蜂蜜对细胞应激反应和抗氧化能力的影响.
主要方法:
- 使用生物反应器采用动态细胞培养模型.
- 在紫外线暴露之前,SSC和HFF1被预处理1%的蜂蜜48小时.
- 实时定量聚合酶链反应 (RT-qPCR) 用于分析基因表达,包括Wnt信号和抗衰老标志物.
- 测量了氧化释放和总抗氧化能力.
主要成果:
- 在SSC中,蜂蜜预处理增强了多能性标记物 (八聚体结合转录因子4 (Oct4);SRY盒转录因子2 (Sox2)) 和减少衰老标记物 (p16,p21,p53).
- 在紫外线损坏的SSC中,蜂蜜显著增加了Wnt3a的表达.
- 在纤维细胞中,蜂蜜上调了热冲击蛋白70 (Hsp70) 和氨酸合成酶2 (HAS2),同时降低了caspase-8 (CASP8) 的调节.
结论:
- 蜂蜜通过调节多能性和老化相关基因,保护皮肤干细胞免受紫外线诱导的衰老.
- 蜂蜜影响Wnt信号传递,这表明蜂蜜在调节干细胞分化和维持皮肤平衡方面发挥了作用.
- 蜂蜜在纤维细胞中对Hsp70的升级增强了细胞应激反应和抗紫外线损伤的稳定性.
相关概念视频
Stem Cell Culture
6.1K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
6.1K
Embryonic Stem Cells
32.4K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.4K
Embryonic Stem Cells
5.0K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
5.0K
Adult Stem Cells
33.8K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.8K
Induced Pluripotent Stem Cells
28.0K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.0K
Cell Culture
22.4K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
22.4K


