咖啡酸补充剂对人类精子对抗体内诱导的氧化应激的影响: Nrf2分子通路
Laura Liguori1, Cinzia Signorini1, Giulia Collodel1
1Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
Antioxidants (Basel, Switzerland)
|January 28, 2026
概括
咖啡酸 (CAF) 在实验室处理过程中保护人类精子免受氧化应激. 这种抗氧化剂可以提高精子运动性和DNA完整性,可能通过Nrf2通路.
科学领域:
- 生殖生物学 生殖生物学
- 生物化学 生化学
- 在体外受精中进行体外受精.
背景情况:
- 氧化应激 (OS) 显著损害精子功能,这在辅助生殖技术中是一个关键问题.
- 在体外抗氧化剂补充剂提供了一种潜在的策略,可以在实验室程序期间保护性质细胞从OS中获取.
研究的目的:
- 研究咖啡酸 (CAF) 在基底条件和诱导氧化应激下对人类精子质量的保护作用.
- 探索涉及CAF保护作用的潜在分子机制,包括Nrf2通路.
主要方法:
- 人类精液样本用不同度的CAF或过氧化 (H2O2) 进行化,以诱导OS.
- 评估了精子运动性,DNA完整性,青体状况,OS标记物 (F2-异oprostanes) 和基因表达 (Nrf2,Keap1,HO-1).
主要成果:
- 100μM的CAF增强了进步的精子运动性,而不会损害DNA完整性.
- CAF治疗抵消了H2O2诱导的损伤,恢复了精子的运动性,DNA完整性和体状况.
- 在CAF上调节Nrf2和HO-1表达,而在H2O2下调节它们;同时治疗使这些水平正常化,表明Nrf2通路参与.
结论:
- 咖啡酸 (CAF) 显示出人类精子对在体外处理过程中遇到的氧化应激的显著保护潜力.
- 补充CAF可以改善精子的关键参数,并通过对Nrf2信号通路的调节来发挥其作用.
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