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金属合酶取物作为一种海洋抗氧化剂,用于稳定DHA纳米乳液
Sakhi Ghelichi1, Behdad Shokrollahi Yancheshmeh1, Mona Hajfathalian1
1Research Group for Bioactives-Analysis and Application, National Food Institute, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
Antioxidants (Basel, Switzerland)
|January 28, 2026
概括
宏藻提取物显示出作为天然化剂的潜力,可以稳定多可萨赫萨酸 (DHA) 纳米乳液,防止氧化. 虽然它们是有效的,但它们在防止金属诱导的降解方面不如合成EDTA那么有效.
科学领域:
- 食品科学与技术 食品科学与技术
- 海洋生物技术 海洋生物技术
- 营养生物化学 营养生物化学
背景情况:
- 作为一种必需的欧米茄-3脂肪酸,多可萨赫萨酸 (DHA) 在纳米乳液中容易氧化.
- 在纳米乳液系统中,较大的界面面积和过渡金属离子加剧了氧化.
- 为DHA纳米乳液开发天然,有效的稳定剂对于营养应用至关重要.
研究的目的:
- 研究藻提取物作为稳定DHA丰富纳米乳液的天然化剂的有效性.
- 为了比较宏提取物对金属诱导氧化与合成化剂 (如EDTA) 的保护作用.
- 评估藻提取物对DHA纳米乳液物理稳定性和氧化标记物的影响.
主要方法:
- 使用Alcalase®进行序列酶-处理提取宏化合物.
- 提取物质的特征,包括Fe2+合活性,蛋白质含量和总化合物.
- 配制DHA纳米乳液,包括不同度的宏藻提取物,并与EDTA和对照组进行比较.
- 评估纳米乳液的物理稳定性 (滴子大小,泽塔潜力) 和氧化稳定性 (氧化物形成,多科菲醇耗尽,挥发性化合物).
主要成果:
- 一种Alcalase®提取物表现出显著的Fe2+合活性 (IC50 = 1.22 mg/mL),含有10.11%的蛋白质和~17μg的GAE/mL基.
- 宏藻提取物保持了纳米乳液的物理稳定性 (滴粒大小~77-80 nm,泽塔电位 -17~19 mV) 并显示了度依赖的界面吸附.
- 提取物处理减缓了氧化物形成,延迟了托科菲醇的耗尽,并将挥发性氧化产品减少了高达40%,但EDTA提供了优越的抗氧化剂保护.
结论:
- 宏藻提取物作为天然的,清洁标签的化剂,有望缓解DHA纳米乳液中的金属催化氧化.
- 这些天然化剂增强了DHA纳米乳液的氧化稳定性,提供了一个可持续的替代品.
- 虽然藻提取物提供了显著的稳定性,但像EDTA这样的合成化剂在严重的抗氧化条件下仍然更有效.
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