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溶解银纳米颗粒通过部分展开蛋白质导致tRNA结合增强来调节内皮单细胞激活聚II (EMAP II)
Lesia Kolomiiets1, Paulina Szczerba1, Wojciech Bal1
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland.
International journal of molecular sciences
|January 28, 2026
概括
银纳米粒子 (AgNPs) 与内皮单细胞激活聚二 (EMAPII) 相互作用,破坏其结构的稳定. 这种相互作用令人惊地增强了EMAP II.
科学领域:
- 生物医学纳米技术 生物医学纳米技术
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 金属纳米粒子 (NPs),特别是银色纳米粒子 (AgNPs),由于其抗菌性能,在生物医学应用中被广泛使用.
- 由于银离子 (Ag+) 向蛋白质中的囊类残留物,AgNP可以表现出毒性.
- 内皮单细胞激活聚二 (EMAPII),AIMP1的蛋白质片段,含有易受Ag+相互作用的表面暴露的囊蛋白.
研究的目的:
- 研究AgNPs,EMAP II和tRNA之间的相互作用.
- 了解AgNP溶解如何影响EMAP II-tRNA结合.
- 阐明Ag+对EMAP II的结构影响及其功能后果.
主要方法:
- 紫外线和光谱学用于研究AgNP溶解和EMAP II-tRNA相互作用.
- 异核1H-15N HSQC光谱带有磁性探针,用于绘制AgNP上的EMAP II结合位点.
- 对EMAP II的结构分析,以评估变性效应.
主要成果:
- 从AgNP中释放的Ag+离子在EMAP II上与暴露的半氨酸结合,导致部分变性.
- 这种结构不稳定性意外地增加了tRNA与EMAP II的亲和力,降低了解离常数 (Kd).
- 这种EMAP II/tRNA/AgNP相互作用是由Mg2+离子和TCEP等醇保护剂调节的.
结论:
- 通过Ag+与氨酸残留物结合,AgNP可以改变EMAP II的结构和功能.
- 观察到的tRNA对Ag+结合EMAP II的亲和力增强表明了复杂的生物影响.
- 了解这些相互作用对于评估AgNP安全性和优化其在生物医学环境中的使用至关重要.
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