在大鼠下缺陷模型中,通过无脚手架的三维构造人类牙干细胞的增强骨再生
Monika Nakano1, Yasuyuki Fujii1, Yuri Matsui-Chujo1,2
1Department of Oral and Maxillofacial Surgery, Tokyo Women's Medical University, 8-1, Kawadacho, Shinjuku-ku, Tokyo 162-0054, Japan.
International journal of molecular sciences
|January 28, 2026
概括
从人类牙髓干细胞 (DPSCs) 生物3D打印的结构表明,它有望在缺陷中再生骨. 这种无支架的方法为大面部重建提供了潜在的新解决方案.
科学领域:
- 再生医学是一种再生医学.
- 生物材料工程 生物材料工程
- 口腔和牙面部外科手术
背景情况:
- 面骨缺陷显著影响功能和美学,目前的治疗方法,如自生骨移植和人工材料都有局限性.
- 来自细胞球体的无脚手架结构的生物3D打印为骨再生提供了一个有希望的替代方案.
- 人类牙纸干细胞 (DPSC) 是再生疗法的可行细胞来源.
研究的目的:
- 为了研究无脚手架的骨质生成潜力,Bio 3D打印了人类牙纤维干细胞 (DPSC) 构造在老鼠下缺陷模型中.
- 评估DPSC球体在促进下骨再生中的有效性.
- 评估使用生物3D打印DPSC构造用于大面部重建的可行性.
主要方法:
- 人类DPSC被隔离并培养成球体,然后用生物3D打印机组装成3D结构.
- 球体和单层培养物被分析为干细胞和早期骨质原生标志物基因表达.
- 3D DPSC构造被移植到免疫缺陷大鼠的下缺陷中,在八周后评估了骨再生.
主要成果:
- 与单层培养相比,DPSC球体表现出较高的干细胞和骨质原生标记的mRNA表达.
- 放射和微CT分析显示,3D构造组的骨体积和矿物密度显著增加.
- 组织学和免疫组织化学分析证实了从移植的DPSC中获得的骨质细胞形成新的骨.
结论:
- 生物3D打印,无脚手架的DPSC结构在临床前模型中有效促进下骨再生.
- 这项技术提供了一种有前途的基于细胞的策略,用于解决大面骨缺陷.
- 使用DPSC的无脚手架3D生物打印是未来大面部重建应用的新方法.
相关概念视频
Stem Cell Therapy for Tissue Regeneration
4.7K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.7K
Adult Stem Cells
33.8K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.8K
Embryonic Stem Cells
32.4K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.4K
Induced Pluripotent Stem Cells
28.0K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.0K
Lumber Defects
509
Lumber defects, which can affect both the appearance and structural integrity of wood, include a variety of growth and manufacturing flaws. Growth defects such as knots and knotholes occur where branches were once attached to the tree trunk, with knotholes forming when these knots fall out. Other natural defects include decay and insect damage, which compromise the wood's strength and durability.
Shakes are minor fractures that run along or across the wood's annual rings, while wane is...
Shakes are minor fractures that run along or across the wood's annual rings, while wane is...
509
Bone Cells and Tissue
8.3K
Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
8.3K


