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在Neolamarckia cadamba中的LBD基因家族的全基因组识别和表达分析

Chuqing Cai¹, Linhan Tang¹, Guichen Jian¹

¹Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.

International journal of molecular sciences

|January 28, 2026

概括

这项研究在奇迹树Neolamarckia cadamba中确定了65个侧面器官边界域 (LBD) 基因. 这些ncLBD基因在植物发育和应激反应中表现出多种表达和作用.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

侧面器官边界域 (LBD) 蛋白质是关键的植物特异性转录因子,参与生长,发育和应激反应.
快速生长,具有药用价值的Neolamarckia cadamba树,之前缺乏LBD基因识别.

研究的目的:

在Neolamarckia cadamba中对LBD基因家族进行全基因组识别和表征.
分析NcLBD基因的遗传关系,分布和调控元素.
在各种条件下研究NcLBD基因的表达模式.

主要方法:

全基因组生物信息学分析用于LBD基因鉴定.
遗传学分析,图案丰富和蛋白质相互作用网络分析.
用于表达特征的RNA测序 (RNA-seq) 和定量实时PCR (qRT-PCR).

主要成果:

在Neolamarckia cadamba中确定了65个NcLBD成员,分为两个分类和七个组.
在NcLBD基因中发现染色体分布不均,并列重复和细分重复.
与MYB相关的和WRKY转录因子结合基因在NcLBD促进体中得到显著丰富.
在各个组织和在激素/无机压力下观察到明显的表达模式.
NcLBD3,NcLBD37和NcLBD47在血管细胞中表达高,并受到寒冷,干旱和盐分压力的诱导.

结论:

这项研究提供了Neolamarckia cadamba中LBD基因家族的综合基因组范围分析.
这些发现为未来研究NcLBD基因的特定生物功能奠定了基础.
确定了可能参与无生物应激耐受性和血管发育的关键NcLBD基因.

关键词:

LBD基因家族的基因家族.新马拉基亚 (Neolamarckia cadamba) 是一种新马拉基亚.无生物压力是无生物压力.表达方式表达方式表达模式

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