来自发酵食品的乳酸菌无细胞超剂的潜力,以控制金黄色葡萄球菌的生长和生物膜的发展
Lena Ilieva1,2, Vesselin Baev3, Mariana Marhova1
1Department of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv, 24 Tsar Asen Str., 4000 Plovdiv, Bulgaria.
International journal of molecular sciences
|January 28, 2026
概括
来自保加利亚turshiya的两种乳酸细菌菌株表现出强大的抗生物膜活性对金黄色葡萄球菌. 他们的稳定后生物产品提供了一种新的,节约抗生素的策略来对抗慢性感染.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 基因组学就是基因组学.
背景情况:
- 黄金葡萄球菌生物膜是持续性感染和抗菌素耐药性的主要原因.
- 乳酸细菌 (LAB) 生产具有潜在治疗应用的抗菌化合物.
- 传统的发酵食品是新型LAB菌株的丰富来源.
研究的目的:
- 为了研究两种LAB菌株L. plantarum IZITR_24和L. paraplantarum IZITR_13的抗葡萄球菌和抗菌膜疗效,这些菌株是从保加利亚制蔬菜 (turshiya) 中分离出来的.
- 通过全基因组测序 (WGS) 和功能测试来描述这些菌株的抗菌武器库.
- 评估这些LAB菌株作为抗S. aureus生物膜的后生物制剂生产者的潜力.
主要方法:
- 全基因组测序 (WGS) 用于识别抗微生物基因.
- 功能性测试以确定最小生物膜抑制度 (MBIC) 和最小抑制度 (MIC).
- 扫描电子显微镜 (SEM) 和共聚焦激光扫描显微镜 (CLSM) 用于可视化生物膜破坏和细胞膜透.
主要成果:
- 在这两种菌株中,WGS确定了保留的植物菌素,IZITR_13也具有小儿菌素和菌素A.的基因.
- 这两种菌株都表现出剂量依赖的S. aureus生长和生物膜形成的抑制.
- 显微镜检查证实了S. aureus生物膜的显著破坏和细胞膜透性的增加.
结论:
- L. plantarum IZITR_24和L. paraplantarum IZITR_13是细菌素的有希望的来源和有效的后生物生产者.
- 基因组第一方法有效预测LAB菌株的抗菌能力.
- 这些LAB衍生后生物提供了针对性,抗生素节约策略,对抗持久的S. aureus生物膜.
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