改变的环境通过扰乱细胞Mg2+平衡来限制结肠直肠HT-29球体生长
Nattida Kampuang1, Pongsakorn Lapchock1, Tanida Treerattanakulporn1
1Department of Medical Sciences, Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand.
International journal of molecular sciences
|January 28, 2026
概括
结肠直肠癌 (CRC) 球体易受 (Mg2+) 不平衡的影响,表现为生长受损和恒温. 准Mg2+运输为CRC提供了一个潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 不调节的 (Mg2+) 恒温与结直肠癌 (CRC) 有关.
- 细胞外Mg2+在CRC瘤微环境中的作用尚未完全理解.
研究的目的:
- 调查持续低和高细胞外Mg2+对结直肠癌 (CRC) 球状体 (SP) 增长和Mg2+恒温的影响.
- 分析Mg2+的流量,运输体表达,活力和分子变化,以应对Mg2+的压力.
主要方法:
- 使用的HT-29 CRC球体 (SPs) 在低或高细胞外Mg2+环境中培养.
- 评估了Mg2+流量,Mg2+运输体的表达 (例如,暂时受体潜力美拉斯 (TRPM) 6),细胞活力,细胞亡,自和/氧化蛋白质变化.
主要成果:
- 低和高2+都破坏了SP架构的稳定性,降低了生命力,并诱导了亡和自.
- 与2D培养相比,SPs表现出更大的脆弱性,受损的Mg2+流入和受损的传送器调节.
- 改变的酸化和TRPM6/7通道的氧化增加抑制了它们的活性,导致功能性Mg2+缺乏,即使在高Mg2+条件下.
结论:
- 结肠直肠癌 (CRC) 球状体非常容易受到Mg2+不平衡的影响,原因是输送器恒常性受损,Mg2+通道受阻.
- 这些发现凸显了CRC中可向的代谢脆弱性.
- 局部Mg2+调制为CRC治疗提供了潜在的治疗途径.
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