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相关概念视频

Nucleosome Remodeling02:54

Nucleosome Remodeling

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Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.2K
ATP Yield01:31

ATP Yield

78.9K
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle).
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
78.9K
Chromatin Packaging02:21

Chromatin Packaging

22.1K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
22.1K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.8K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
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Epigenetic Regulation01:46

Epigenetic Regulation

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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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相关实验视频

Updated: Jan 29, 2026

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
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Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

Published on: October 25, 2014

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如何ATP依赖的染色体重塑复合体调节脊椎动物胚胎发育

Hejie Wang1, Gulinigaer Anwaier2, Shengbin Bai1

  • 1Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830017, Xinjiang, China.

International journal of molecular sciences
|January 28, 2026
PubMed
概括

对基因表达至关重要的ATP依赖的染色质重塑剂在胚胎发育和干细胞维护中至关重要. 了解这些复杂物有助于解决发育缺陷和推进再生医学.

关键词:
染色体重塑复杂物 染色体重塑复杂物胚胎发育过程中的胚胎.胚胎基因组激活活动表观遗传修饰 表观遗传修饰谱系规范的规格 谱系规范的规格

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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

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Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples
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Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples

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相关实验视频

Last Updated: Jan 29, 2026

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
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Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

Published on: October 25, 2014

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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

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Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples
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Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples

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科学领域:

  • 分子生物学分子生物学
  • 发展生物学 发展生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 依赖ATP的染色体重塑复合体是通过修改染色体结构来调节基因表达的关键调节者.
  • 这些复合体被分为四个家族 (SWI/SNF,ISWI,CHD,INO80) 并执行诸如核酶体滑动和驱逐等功能.
  • 它们涉及至关重要的过程,包括DNA修复,瘤生成,器官生成和胚胎发育.

研究的目的:

  • 提供对依赖ATP的染色质重塑复合物的系统概述.
  • 详细说明它们的分类,保存机制和早期胚胎发生中的功能.
  • 讨论它们在胚胎干细胞维护中的作用以及对发育生物学的影响.

主要方法:

  • 关于低输入蛋白质组学和发育生物学近期进展的文献综述.
  • 对染色体重塑复杂分类和机制的系统分析.
  • 讨论脊椎动物胚胎发育中的功能作用,包括哺乳动物和非哺乳动物模型.

主要成果:

  • 染色体重塑剂对于胚胎基因组激活,血统规范和哺乳动物的干细胞命运至关重要.
  • 在像Xenopus laevis这样的非哺乳动物模型中,它们在胃化和神经化过程中对细胞命运决定至关重要.
  • 这些复合体参与了从胚胎细胞形成到器官生成前阶段的基本过程.

结论:

  • 依赖ATP的染色体重塑复合体是基因表达的保守调节者,对胚胎发育至关重要.
  • 它们的功能对于理解发育缺陷至关重要,并且对再生医学具有前景.
  • 对这些复合体的进一步研究可以阐明细胞命运决定和干细胞维护的机制.