相关实验视频

Updated: Jan 29, 2026

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

来自Lonomia obliqua的毒素衍生的蛋白质调节细胞骨调节器和人类冠状细胞的炎症反应

Miryam Paola Alvarez-Flores^1,2,3, Amanda Teixeira de Melo¹, Renata Nascimento Gomes¹

¹Centre of Excellence in New Target Discovery (CENTD), Butantan Institute, São Paulo 05503-900, Brazil.

International journal of molecular sciences

|January 28, 2026

概括

洛诺米亚斜毛皮提取物及其蛋白质通过调节细胞中的炎症反应和细胞骨通路,显示出对骨关节炎的潜力,提供新的治疗途径.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

骨关节炎 (OA) 是一种退行性关节疾病,随着软骨的逐渐损失和炎症.
目前的OA疗法无法阻止疾病的进展,需要新的治疗方法.
冠状细胞在OA的发病过程中发挥着关键作用,对IL-1β.等炎症信号作出反应.

研究的目的:

为了研究Lonomia obliqua毛发提取物 (LOCBE) 和其重组蛋白 (rLOPAP,rLOSAC) 对人体红细胞的影响.
评估它们在调节炎症途径和与OA相关的细胞骨调节者的潜力.

主要方法:

人类冠状细胞用IL-1β,LOCBE,rLOPAP或rLOSAC进行治疗.
分析了细胞因子分泌 (IL-6,IL-8),蛋白质表达 (β-catenin,RhoA,Rab9,Rac-1),以及NF-κB转位.
评估了线粒体活动.

主要成果:

洛贝和rLOPAP诱导了中度的IL-6和IL-8分泌.
LOCBE证明了细胞保护作用和降低β-catenin,RhoA和Rab9的表达.
在炎症下,rLOPAP增强了线粒体活性和Rac-1,而rLOSAC降低了RhoA/Rac-1并增加了β-catenin.

结论:

LOCBE,rLOPAP和rLOSAC调节细胞中的炎症反应和细胞骨动力学.
这些化合物通过向关键的OA进展机制,显示出作为治疗关节炎的治疗途径的希望.

关键词:

一个IL-1ββ.罗姆尼亚罗姆尼亚罗姆尼亚一个Rac-1一个Rac-1.在RhoAAA 冠状腺细胞 (chondrocytes) 是一种体内细胞.细胞因子细胞因子细胞骨通路中的细胞骨通路.炎症反应可能是炎症反应.这是一种β-catenin.

更多相关视频

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Published on: July 18, 2017

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

相关概念视频

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

Cytoskeletal Accessory Proteins

Cytoskeletal Accessory Proteins

The cytoskeleton is an essential cell component that plays several structural and functional roles. However, the filaments that make up the cytoskeleton cannot function independently and depend on the accessory or ancillary proteins to effectively carry out their function. Accessory proteins associate with cytoskeletal filaments and their monomers, aiding filament formation and function. They also help in the cross-communication among cytoskeletal filaments. Cytoskeletal accessory proteins are...

Cytoskeletal Proteins in Bacteria

Cytoskeletal Proteins in Bacteria

Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...

Inflammatory Response II: Inflammatory Exudate and Tissue Repair

Inflammatory Response II: Inflammatory Exudate and Tissue Repair

The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...