电离辐射诱导细胞外陷从巨细胞释放
Yongchan Lee1, Monowar Aziz1,2, Ping Wang1,2
1Center for Immunology and Inflammation, The Feinstein Institutes for Medical Research, 350 Community Dr., Manhasset, NY 11030, USA.
International journal of molecular sciences
|January 28, 2026
概括
暴露于电离辐射会触发巨细胞通过细胞灭绝,细胞死亡过程释放细胞外陷 (METs). 针对这些辐射诱导的METs可能提供新的策略来减少组织损伤.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 辐射生物学 辐射生物学
背景情况:
- 巨细胞是对抗病原体防御的重要先天性免疫细胞.
- 电离辐射可以破坏巨细胞的功能,并加剧组织损伤.
- 巨细胞外陷 (METs) 在刺激时释放,并在免疫反应中发挥作用.
研究的目的:
- 为了调查电离辐射暴露是否诱导巨细胞释放细胞外陷.
- 阐明辐射诱导的MET形成背后的机制.
- 探索针对METs减轻辐射诱导组织损伤的潜力.
主要方法:
- 从C57BL/6小鼠收集的腹巨细胞.
- 巨细胞暴露在5 Gy电离辐射下.
- 使用免疫光检测用于素化素H3和无细胞DNA测量,评估MET形成.
- 分析了细胞死亡途径,包括由Gasdermin D (GSDMD) 介导的热.
- 研究了基氨酸减弱酶 (PAD) 2和4在MET形成中的作用.
主要成果:
- 电离辐射诱导显著的巨细胞死亡通过GSDMD介导的灭.
- 热导致了自杀性和重要的METosis.
- 辐射暴露增加了MET的形成,由增加的素基因素H3和细胞外DNA证明.
- PAD 2和4对于辐射诱导的MET产生至关重要.
结论:
- 电离辐射诱导巨细胞通过GSDMD激活和热的细胞外陷形成.
- 在暴露于辐射后,PAD酶对METosis至关重要.
- 向METs为管理辐射诱导的组织损伤提供了潜在的治疗途径.
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