在生物功能化Ti6Al4V合金上增强细胞粘附:从Spirulina platensis微藻中固定蛋白质和生物质
Maria Fernanda Hart Orozco1, Rosalia Seña2, Lily Margareth Arrieta Payares3
1Department of Mechanical Engineering, Universidad del Norte, Barranquilla 081007, Colombia.
International journal of molecular sciences
|January 28, 2026
概括
用Spirulina platensis生物质或蛋白质提取物生物功能化合金表面可以增强早期细胞粘附. 与蛋白质提取物和对照表面相比,生物质涂层显示出更好的细胞附着.
科学领域:
- 生物材料科学 生物材料科学
- 表面化学 表面化学
- 细胞生物学 细胞生物学
背景情况:
- (Ti) 和其合金对于生物医学植入物至关重要,因为它们具有出色的生物相容性和耐腐蚀性.
- 提高Ti表面的生物功能对于改善植入物性能和骨质整合至关重要.
- 使用天然生物分子进行表面修饰,为改善早期细胞物质相互作用提供了一个有前途的途径.
研究的目的:
- 为了研究Ti6Al4V合金表面与Spirulina platensis (S. platensis) 生物质和蛋白质提取物的生物功能化作用.
- 评估这些表面修饰的形态,化学和生物影响.
- 为了确定最佳的S. platensis成分和度,以增强细胞粘附.
主要方法:
- Ti6Al4V表面使用皮拉纳溶液被激活,并用3-aminopropyltriethoxysilane (APTES) 进行化.
- S. platensis生物质和蛋白质提取物被固定在化表面上.
- 使用扫描电子显微镜 (SEM),ICP-MS,EDS和FTIR进行表面表征.
- 进行了细胞粘附测试,以量化早期的细胞附着.
主要成果:
- 证实了S. platensis成分的成功结合,由,和氧含量增加表明.
- 生物质功能化的表面显示出更高的和氧气,而蛋白质涂层的表面显示出富含的特征.
- 与对照组相比,在生物功能化的表面上观察到提升的早期细胞粘附.
- 用5g/L的S. platensis生物质处理的表面表现出明显更大的细胞附着比蛋白质处理和对照表面.
结论:
- 斯普拉丁斯生物质和蛋白质提取物差异调节Ti6Al4V表面化学和生物反应.
- 使用S. platensis,特别是生物质的生物功能化,有效地增强了植入物上的早期细胞粘附.
- 使用特定的微藻成分定制生物功能化策略对于优化植入物生物性能至关重要.
相关概念视频
Adhesion
44.1K
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
Capillary action is a result of water’s adhesive tendencies. When a narrow...
44.1K
Cell Adhesion in Plants
3.3K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
3.3K
Laminins are the Adhesive Proteins of Basal Lamina
3.2K
Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
3.2K
Immunoglobulin-like Cell Adhesion Molecules
4.4K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
4.4K
Cell Adhesion Molecules - Types and Functions
9.3K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved...
CAM Families
The Integrin family of proteins is primarily involved...
9.3K
Self-Evaluation: Self-Enhancement and Self-Verification
5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K


