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转录组分析揭示了来自Rhodomyrtus tomentosa的两个多糖的免疫调节作用
Dingjin Li1,2, Qiuxia Duan1, Wan Zunairah Wan Ibadullah2
1Guangxi Key Laboratory of Health Care Food Science and Technology, School of Food and Biological Engineering, Hezhou University, Hezhou 542800, China.
Foods (Basel, Switzerland)
|January 28, 2026
概括
来自Rhodomyrtus tomentosa果的两种多糖,RTP-1和RTP-2,对巨细胞表现出免疫刺激作用. RTP-2显示出增强巨细胞功能和调节免疫基因表达的能力更强.
科学领域:
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
- 自然产品 化学 化学
背景情况:
- 罗多米尔特斯门果 (Rhodomyrtus tomentosa berry) 含有各种多糖类,具有潜在的生物活性.
- 这些多糖的免疫调节性质尚不清楚.
- 研究这些特性可以揭示免疫支持的新功能成分.
研究的目的:
- 在RAW264.7巨中评估两种不同的Rhodomyrtus tomentosa多糖的免疫刺激活性 (RTP-1和RTP-2).
- 通过转录组分析阐明其影响背后的分子机制.
- 为了确定这些多糖类作为免疫增强剂的潜在应用.
主要方法:
- 使用中性红色试验评估巨细胞细胞功能.
- 通过Griess测定和光探针测量了氧化 (NO) 和活性氧物种 (ROS) 的产生.
- 使用ELISA进行量化细胞因子水平 (TNF-α,IL-6,IL-1β).
- 分析了RNA-seq数据与加权基因共同表达网络分析 (WGCNA).
- 使用定量实时PCR (RT-qPCR) 验证了关键基因表达变化.
主要成果:
- 无论是RTP-1还是RTP-2,都增强了巨细胞化,NO/ROS产生和细胞因子分泌.
- RTP-2激活了METurquoise联合表达模块 (222个枢纽基因),与髓细胞免疫调节和先天免疫信号相关.
- RTP-1主要与MECyan模块 (49个枢纽基因) 相关.
- 在RTP-2组中,RT-qPCR证实了Dram1和Bmf1的显著上调和Bnip3的下调.
- 在实验室中,RTP-2显示出更明显和更连贯的巨细胞刺激特征.
结论:
- Rhodomyrtus tomentosa 多糖,特别是RTP-2,具有显著的免疫刺激性质.
- RTP-2有效地激活巨细胞,影响关键的免疫路径.
- 作为功能性食品或治疗药物的向免疫刺激成分,RTP-2显示出希望.
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