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EjMYB15通过升级抗氧化酶基因提高了收获后洛卡特水果的耐寒性

Weiqi Liang¹, Jiahui Wan¹, Jing Lin¹

¹Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Foods (Basel, Switzerland)

|January 28, 2026

概括

黑色素治疗通过增加EjMYB15表达,减少了loquats的冷却损伤. 这种转录因子激活抗氧化基因,增强耐寒性,提高存储期间的水果质量.

科学领域:

植物生理学植物生理学
分子生物学分子生物学
收获后生物学收获后生物学

背景情况:

鱼在冷藏期间容易受到冷却损伤 (CI),造成重大经济损失.
外源性黑素 (MT) 通过调节活性氧物种 (ROS) 代谢来减轻CI,但其分子机制尚不清楚.

研究的目的:

为了阐明MT减轻收获后鱼CI的分子途径.
研究MYB转录因子 (TFs) 在调节抗氧化酶基因中的作用.

主要方法:

评估CI严重程度,细胞膜透性和水果质量参数 (坚硬度,颜色值).
通过表达分析,亚细胞局部化和交换激活试验,鉴定和描述了冷诱导的MYB TF (EjMYB15).
通过EMSA和DLR测定证实了EjMYB15与抗氧化基因促进体 (EjCAT1,EjCAT2,EjGST1,EjGST2) 的结合.

主要成果:

MT治疗显著降低了CI的严重程度,并改善了瓜水果的质量.
通过MT治疗,EjMYB15的表达被上调,它作为核转录激活剂起作用.
EjMYB15直接激活了抗氧化酶基因 (EjCAT1,EjCAT2,EjGST1,EjGST2) 的转录.

结论:

EjMYB15通过增强ROS清理能力,积极调节瓜水果的耐寒性.
这项研究阐明了MYB TF介导的MT调节途径,以缓解CI.
这些发现为使用MT减轻水果收获后CI提供了理论支持.

关键词:

转录因子MYB转录因子在ROS清理垃圾.寒冷的伤害伤害的伤害洛克瓦特 (loquat) 是一个很小的鱼类.黑激素是什么意思黑激素是什么意思

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