植物性蛋白质生物基因与转氨酸酶交叉连接:3D打印能力和分子洞察力从NMR和同步子-FTIR
Jaksuma Pongsetkul1, Sarayut Watchasit2, Tanyamon Petcharat3
1School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Foods (Basel, Switzerland)
|January 28, 2026
概括
转胺酶 (TGase) 通过改善交联和网络形成来增强用于3D食品打印的植物蛋白生物墨水. 这提高了可持续的功能性食品的可打印性,质感和结构完整性.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物聚合物科学 生物聚合物科学
- 材料科学 材料科学 材料科学
背景情况:
- 对于可持续的,功能性的植物性食品的日益增长的需求需要先进的加工技术,如3D食品打印.
- 食品3D打印需要具有精确控制的质和结构性质的生物墨水才能成功制造.
- 植物蛋白是有前途的生物油墨成分,但通常需要修改以实现所需的功能.
研究的目的:
- 研究转谷氨酸酶 (TGase) 对植物蛋白生物墨水的结构功能关系的影响,这些生物墨水来自豆,豆,豆和大豆.
- 评估TGase如何影响这些植物蛋白生物油墨的蛋白质交联,网络形成,质性质和纹理特征.
- 确定TGase作为一种提高植物蛋白适用于食品3D打印应用的工具的潜力.
主要方法:
- 使用TNBS试验对蛋白质交联的定量分析.
- 频谱技术 (1H-NMR,同步射线FTIR) 来确认键形成和结构变化.
- 用于分析蛋白质聚合和聚合物形成的SDS-PAGE.
- 扫描电子显微镜 (SEM) 用于可视化蛋白质网络结构.
- 风湿学测量以评估粘性弹性和剪切稀释行为.
- 纹理分析用于评估硬度和性等机械性能.
主要成果:
- TGas治疗显著增加了蛋白质交叉连接,以剂量依赖的方式 (27.46-64.57%),蛋白质来源之间有显著差异 (大豆和豆的反应性最高).
- 证实了e-γ-glutamyl) lysine键的形成和TGase诱导的二次蛋白质结构的改变 (α-螺旋减小,β-叶的丰富),表明了网络的发展.
- SDS-PAGE揭示了TGase介导的聚合和高分子量聚合物的形成,特别是在大豆蛋白中.
- 与对照组相比,SEM图像显示TGase处理的生物墨水中的蛋白质网络更密集,更连续.
- 在所有TGase处理的生物油墨中观察到增强的粘性弹性和剪切稀释行为,改善了打印能力和稳定性.
- 在硬度,弹性,凝聚性和性方面有显著的改进,大豆和豆蛋白显示出最实质性的改进.
结论:
- 转胺酶 (TGase) 是一种有效的交联剂,用于加强生物油墨中的植物蛋白质网络.
- TGas处理显著改善了植物蛋白质基生物墨水的风湿性质,可打印性,结构完整性和质感.
- 该研究强调了对TGase的反应中的蛋白质特异性变异,为为特定的3D食品打印应用量身定制生物墨水提供了见解.
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