在从临床和肠道样本中分离的抗万科米辛抗性肠球菌中病毒性因素的分布
1Department of Microbiology and Virology, Faculty of Pharmacy, Medical University-Pleven, 1st St. Kliment Ohridski Str., 5800 Pleven, Bulgaria.
Microorganisms
|January 28, 2026
概括
这项研究发现,从临床和肠道样本中,ACM和ESP等毒性因子在抗旺素的肠球菌 (VRE) 中常见. 它们的患病率在临床和肠道Enterococcus faecium之间没有显著差异.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 遗传学 是一个遗传学.
背景情况:
- 肠球菌是重要的病原体,其毒性因素有助于感染和殖民.
- 抗万科胺素的肠球菌 (VRE) 构成了日益严重的临床挑战.
- 了解VRE毒性决定因素对于对抗感染至关重要.
研究的目的:
- 调查VRE.中编码毒性因子的基因的流行情况.
- 为了比较这些基因在临床和肠道样本中的VRE中的分布.
- 在VRE中识别主导性毒性基因及其组合.
主要方法:
- 维特克2系统用于163个VRE分离物种的物种识别 (94个临床,69个肠道).
- 多重复合PCR检测菌素耐药基因 (vanABCDMN) 和毒性因子基因 (ace/acm, asa1, esp, efaA, cylA, gelE, hyl).
- 费舍尔精确测试用于临床和肠道E. faecium.之间基因流行率的统计比较.
主要成果:
- 在163个VRE分离物中,有107个含有毒性因子.
- 最常见的毒性基因是acm和esp.
- 在临床和肠道E. faecium.之间没有观察到病毒性基因患病率的显著差异.
- 在94个隔离物中发现了acm和esp的组合.
结论:
- VRE含有各种各样的毒性决定因素.
- 基因acm和esp在VRE中经常同时存在并占主导地位.
- 病毒性因子的流行率在临床和肠道VRE中是相似的,这表明传播和殖民的潜力.
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