为临床转化优化阴道微生物组分析:对样本存储方法和阴道特异性16S rRNA基因数据库的比较评估
Alishum Ali1,2, Jeffrey A Keelan3,4, Blagica Penova-Veselinovic4,5
1Trace and Environmental DNA (TrEnD) Laboratory, School of Molecular Life Sciences, Curtin University, Perth, WA 6102, Australia.
Microorganisms
|January 28, 2026
概括
使用标准化方法进行阴道微生物组分析可以可靠地评估早产风险 (PTB). 艾米斯稳定片和专用数据库 (VagDB) 提高了PTB风险评估的诊断准确性.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 产科 产科 产科 产科 产科
背景情况:
- 阴道微生物组的组成与早产 (PTB) 风险有关.
- 16S rRNA基因分析识别了与PTB相关的特定阴道社区状态类型 (CST).
- 标准化预分析协议对于诊断概况至关重要.
研究的目的:
- 评估用于16S rRNA微生物分析的两个阴道拭子存储方法.
- 为了验证阴道特定的16S rRNA基因数据库 (VagDB),以进行增强的注释.
- 评估用于PTB风险评估的阴道微生物组分析的可靠性.
主要方法:
- 两种储存方法 (干燥/立即冷 vs. 艾米斯稳定/冷) 用22名高PTB风险女性的配对拭子进行了比较.
- 进行了16S rRNA基因 (V4) PCR和Illumina测序.
- 评估了多样性,组成,CST分配和DNA产量;使用VagDB进行注释.
主要成果:
- 艾米斯稳定样本的DNA显著增加 (p = 0.003),但没有改变微生物多样性或社区结构.
- VagDB 改进了物种级别的分辨率.
- 根据参与者和CST进行聚类是稳健的 (p < 0.001),无论存储如何,CST一致性> 90%.
结论:
- 在8-72小时制冷窗口的Amies介质中例行采集的阴道拭片产生了可靠的微生物组数据.
- 这些发现支持将阴道微生物组分析纳入临床PTB风险评估.
- 标准化的协议和专用数据库提高了诊断效用.
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