用磁雕塑的微流体用于通过EpCAM表达水平对细胞群的连续流量分离
Zhenwei Liang1, Xiaolei Guo1,2, Xuanhe Zhang1
1Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
Micromachines
|January 28, 2026
概括
这项研究介绍了一种微流体平台,使用软磁条进行基于表面标记水平的连续细胞分离. 它可以进行定量,标签引导的细胞分离,具有高回收率和下游应用的可行性.
科学领域:
- 生物医学工程 生物医学工程
- 微流体学 微流体学
- 细胞分离技术 细胞分离技术
背景情况:
- 磁标记细胞的连续流分离对于研究细胞异质性和支持测试至关重要.
- 现有的方法需要精确控制磁场和细胞标签,以实现有效的分离.
研究的目的:
- 开发一个微流体平台,用于基于表面标记物表达水平的定量,连续流动的细胞分离.
- 建立磁场设计策略,使用软磁元素进行表达水平依赖的细胞分离.
主要方法:
- 利用空间工程软磁带 (SMS) 在微通道中创建侧向磁偏向场.
- 采用COMSOL-MATLAB框架和力等效度量来优化SMS结构参数.
- 实施了三阶段级联络,采集多个出口,以便将细胞分成离散的表达水平子组.
主要成果:
- 成功地将MDA-MB-231,Caco-2和A549细胞分成四个与EpCAM相关的磁性子组 (高,中,低,近负).
- 获得高细胞恢复率 (>90%) 和维持细胞活力 (98.2 ± 1.3%).
- 证明了排序的分数与已知的文学表达趋势保持一致.
结论:
- 开发的微流体平台允许基于表面标记表达的定量,连续流动的细胞分离.
- 使用SMS的磁场设计策略对于下一代磁流体分离系统是有效的.
- 该系统兼容下游应用,包括全血检测和细胞培养.
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