在405nm的近紫外线辐射:对Gram-阳性和Gram-阴性细菌的细菌杀菌效应和没有亚致命条件
Davide Amodeo1, Giulio Pedrazzoli2, Isa De Palma1
1Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.
Pathogens (Basel, Switzerland)
|January 28, 2026
概括
近UVA光 (405nm) 显示出对消毒表面和减少医疗保健相关感染的承诺. 将这种光与透,热应激或氨酸相结合,可显著增强其对各种细菌的抗菌作用.
科学领域:
- 摄影化学和摄影生物学
- 微生物学 微生物学
- 传染病控制和控制传染病
背景情况:
- 医疗相关感染 (HAI) 需要创新的消毒策略.
- 紫外线 (UV) 辐射,包括UVA,UVB和UVC,具有抗菌性质.
- 国内基于紫外线的消毒设备的开发正在增加.
研究的目的:
- 评估近UVA辐射 (405nm) 作为替代消毒技术的有效性.
- 调查亚致死的压力条件 (奥斯摩斯,热) 和光敏感剂对近紫外线光近紫外线光的细菌失活的影响.
- 评估近紫外线辐射对抗HAI的潜力.
主要方法:
- 四种细菌菌株 (S. aureus,E. faecalis,E. coli,P. aeruginosa) 在各种条件下暴露在近紫外线辐射 (405 nm,21 mW/cm2) 下.
- 照射是在亚致死的和非亚致死的压力下进行的,包括透压力,热压力 (4°C和45°C),以及外源光敏的存在.
- 使用log10减量量化了细菌的减量.
主要成果:
- 仅靠近紫外线的光线就实现了对P. aeruginosa (2.4 log10) 的最大降低.
- 与透应激的联合治疗导致所有菌株的细菌显著减少 (平均1.5-5.46 log10).
- 在45°C的热应激对大多数物种有效,而外源性氨酸对S. aureus和P. aeruginosa有很高的有效性.
结论:
- 405nm的近紫外线辐射显示出显著的消毒潜力,特别是在与特定的次致命压力条件相结合时.
- 接近UVA光和外源氨酸的组合产生了对S. aureus和P. aeruginosa产生最实质性的杀菌效应.
- 这种方法为消毒提供了一个有希望的策略,以减轻HAI的传播.
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