脉冲电场技术对乳清蛋白缩物的影响
Elizabeth L Ryan1, Owen M McDougal2
1Biomolecular Sciences PhD Program, College of Arts and Sciences, Boise State University, Boise, ID 83725, USA.
Molecules (Basel, Switzerland)
|January 28, 2026
概括
乳清蛋白缩物 (WPC-80) 的脉冲电场 (PEF) 处理改善了其物理和功能性质,包括降低粘度和颗粒大小,增强溶解性和乳化稳定性,而不会改变蛋白质结构.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物物理学的生物物理.
- 蛋白质化学 蛋白质化学
背景情况:
- 乳清蛋白缩物 (WPC-80) 是一种有价值的乳制品成分.
- 优化WPC-80属性可以增强其应用.
- 脉冲电场 (PEF) 处理是一种非热技术,具有改变食品的潜力.
研究的目的:
- 研究脉冲电场 (PEF) 处理对10% (体积/体积) 乳清蛋白缩物 (WPC-80) 的物理和功能性质的影响.
- 评估PEF处理后粘度,颗粒大小,可溶性,乳化和蛋白质结构完整性的变化.
- 评估使用连续流PEF系统用于WPC-80处理的可扩展性和商业可行性.
主要方法:
- 在三个不同的条件下,将WPC-80复合到10% (m/v) 并用PEF处理.
- 测量PEF处理的乳清溶液的表面粘度和颗粒大小.
- 通过循环二元化 (CD),光和差分扫描热量计 (DSC) 来解决干燥的乳清并评估蛋白质结构完整性.
- 评估功能性质,包括可溶性,可发泡性和乳化.
主要成果:
- PEF处理减少了表面粘度和颗粒大小.
- 通过PEF处理,溶解度和乳化稳定性显著增加.
- 发泡性和分子量概况保持不变.
- 通过CD,光或DSC检测到蛋白质结构没有显著的变化.
结论:
- 使用可扩展的连续流系统,以10% (m/v) 的WPC-80的PEF处理有利于修改物理和功能性质.
- 观察到的可溶性和乳化稳定性的改善表明,WPC-80在各种食品系统中具有更好的适用性.
- 在不损害蛋白质完整性的情况下,PEF为WPC-80修改提供了一种有希望的非热方法.
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