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在生物流体中测定性激素的方法是通过在瓶内进行聚烯酸乙烯薄膜微提取,并与HPLC-MS/MS结合
Francesca Merlo1, Silvia Anselmi1, Andrea Speltini1
1Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy.
Molecules (Basel, Switzerland)
|January 28, 2026
概括
聚卡普罗拉克薄膜在生物样本中提供有效的性激素在小瓶内微提取. 这种绿色方法提供快速提取和可重复使用的薄膜,用于准确的HPLC-MS/MS分析.
科学领域:
- 分析化学 分析化学
- 环境化学环境化学
- 生物化学 生物化学
背景情况:
- 试管内微提取 (IVME) 将样品制备步骤集成到一个单一的设备中.
- 对于IVME应用,正在探索聚烯酸 (PCL) 薄膜.
- 在生物矩阵中确定未结合性激素需要有效的样本准备.
研究的目的:
- 评估PCL聚合物薄膜对未结合性激素的低容量IVME的适用性.
- 用HPLC-MS/MS评估PCL膜在尿液和血清等生物样本中的性能.
- 为了确定基于PCL的IVME方法的效率,可重复使用性和绿色性.
主要方法:
- 使用的PCL聚合物薄膜用于性激素的内方微提取.
- 分析了尿液,牛血清白蛋白 (BSA) 溶液和胎儿牛血清 (FBS) 样本.
- 使用高性能液体染色学-并联质谱法 (HPLC-MS/MS) 进行量化.
- 实施矩阵匹配校准和布拉德福德试验来定量蛋白质.
主要成果:
- 使用乙醇实现了快速提取时间 (10-30分钟) 和令人满意的化.
- 在不同的生物矩阵中获得了良好的恢复率 (55-122%).
- 证明了需要蛋白质矩阵稀释和清洁提取物的洗步骤.
- 报告了检测和定量化的低极限 (0.01-3.0 ng mL-1).
- 证实PCL薄膜可重复使用长达10个周期.
结论:
- 在复杂的生物样本中,PCL膜有效地提取和清除痕迹性激素.
- 使用PCL薄膜的IVME方法遵循绿色样品制备原则.
- 这种技术为生物液体中性激素的常规分析提供了可行的替代方案.
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