使用基因组挖掘技术发现了来自内菌微型红菌FoRo54的新罗萨米辛衍生物
Zhi-Bin Zhang1, Qi Liu1, Guo-Dong Song1
1Key Laboratory of Biodiversity Conservation and Bioresource Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang 330022, China.
Molecules (Basel, Switzerland)
|January 28, 2026
概括
从野生大米中分离出来的一种内菌细菌,Micromonospora rosaria FoRo54,产生了新的抗菌化合物. 基因组分析揭示了其发现新生物活性代谢物的潜力.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 自然产品 化学 化学
背景情况:
- 从Oryza rufipogon中分离出的内生菌,如Micromonospora rosaria FoRo54,代表了新型生物活性化合物的有希望的来源.
- 这种内植物的遗传构成和代谢能力对于了解它们在药物发现中的潜力至关重要.
研究的目的:
- 为了识别和描述内菌FoRo54.4的特征.
- 为了研究二次代谢物生物合成基因集群的FoRo54的基因组.
- 隔离和识别由FoRo54产生的生物活性化合物,并评估它们的抗菌活性.
主要方法:
- 形态特征和16S rRNA基因测序用于细菌识别.
- 使用antiSMASH进行全基因组测序和生物信息分析,用于识别生物合成基因集群 (BGCs).
- 发酵,化合物分离,结构阐明,以及对黄金葡萄球菌的抗菌分析.
主要成果:
- 菌株FoRo54被确定为Micromonospora rosaria,具有7.06 Mb的线性染色体,GC含量为73.8%.
- 基因组挖掘揭示了27个BGC,包括罗萨米辛和卡纳米辛生物合成的BGC,与观察到的抗菌活性相关.
- 四种化合物,包括一种新的罗萨米辛衍生物 (N-甲基罗萨米辛),被分离出来,并显示出对金黄色葡萄球菌的强烈活性.
结论:
- 内生菌的微型单胞菌 (Micromonospora rosaria) FoRo54表现出显著的代谢潜力,产生新的抗菌化合物.
- 基因组洞察力为了解这些代谢物的生物合成和未来基因工程工作提供了基础.
- 这项研究强调了内菌微生物作为发现新抗菌剂的来源的价值.
相关概念视频
02:02Genomics
40.4K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.4K
01:26Drug Discovery: Overview
11.4K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
11.4K
02:30Genomic Imprinting and Inheritance
37.1K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.1K
03:21Genome Size and the Evolution of New Genes
9.1K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.1K
01:31Hybridoma Technology
17.6K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.6K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
15.9K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
15.9K