基于机器学习的图像可比性评估,用于跨平台的流成像显微镜
Zhenhao Zhou1, Sha Guo2, Youli Tian3
1School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China.
Pharmaceuticals (Basel, Switzerland)
|January 28, 2026
概括
流成像显微镜 (FIM) 为生物制药中可见微粒分析提供了一个有希望的替代方案. 这项研究开发了一种策略,用于在不同的FIM仪器中进行一致的粒子计数,以帮助标准化工作.
科学领域:
- 生物制药学分析分析
- 分析化学是一种分析化学.
- 显微镜技术的使用方法
背景情况:
- 生物制药开发需要严格的质量控制,可见微粒是关键属性.
- 目前的药典方法 (光遮蔽,微观粒子计数) 对于先进的分析需求有局限性.
- 流成像显微镜 (FIM) 提供了一个潜在的替代方案,但标准化正在进行中.
研究的目的:
- 评估粒子计数在不同流成像显微镜 (FIM) 平台上的一致性和可转移性.
- 用FIM技术评估使用标准化可见微粒子分析的可行性.
- 探索跨制造商的FIM数据的图像分类一致性.
主要方法:
- 使用聚烯微球标准和静脉注射免疫球蛋白的仪器标准化和一致性评估.
- 来自不同制造商的FIM仪器的并行测试.
- 使用卷积神经网络和UMAP缩小维度的粒子图像分类.
主要成果:
- 开发了一种传输策略,以便在FIM平台上获得一致的粒子计数结果.
- 粒子图像分类在分类中表现出一致性,但突出了跨平台识别挑战.
- 该研究证实了FIM可靠的可见微粒子分析的潜力.
结论:
- 这些发现有助于规范流成像显微镜用于生物制药质量控制.
- 作为一个可靠的分析工具,FIM显示出可靠的潜可见粒子表征.
- 需要进一步开发,以在FIM中完全标准化跨平台图像识别.
更多相关视频
04:09Predicting Treatment Response to Image-Guided Therapies Using Machine Learning: An Example for Trans-Arterial Treatment of Hepatocellular Carcinoma
Published on: October 10, 2018
8.7K
08:58Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
Published on: November 19, 2018
13.0K
相关概念视频
Imaging Biological Samples with Optical Microscopy
10.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
10.1K
Machines
576
Machines are complex structures consisting of movable, pin-connected multi-force members that work together to transmit forces. One example of a machine is the cutting plier, which is used to cut wires by applying forces to its handles. When equal and opposite forces are exerted on the handles of the cutting plier, they cause the cutting edges to come together and apply equal and opposite reaction forces on the wire, which are greater than the applied forces.
A free-body diagram of the...
A free-body diagram of the...
576
Imaging Studies VII: Vascular Imaging
361
DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...
361
Machines: Problem Solving II
663
Machines are complex structures consisting of movable, pin-connected multi-force members that work together to transmit forces. Consider a lifting tong carrying a 100 kg load. It comprises movable sections DAF and CBG linked together with member AB.
663
X-ray Imaging
10.1K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
10.1K
Brain Imaging
727
Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans), magnetic resonance imaging (MRI), functional magnetic resonance imaging (fMRI), and Transcranial Magnetic...
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans), magnetic resonance imaging (MRI), functional magnetic resonance imaging (fMRI), and Transcranial Magnetic...
727
