来自Pyrus ussuriensis的代谢物和抗氧化成分的识别
Ducdat Le1,2, Thientam Dinh1, Soojung Yu2
1College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, 255 Jungangno, Suncheon 57922, Jeonnam, Republic of Korea.
Pharmaceuticals (Basel, Switzerland)
|January 28, 2026
概括
这项研究开发了一种快速超高性能液体染色学-并联质谱法 (UHPLC-MS/MS) 方法,用于识别Pyrus ussuriensis中的抗氧化化合物. 该方法成功地确定了活性成分,突出了植物.
科学领域:
- 植物化学 植物化学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 皮鲁斯乌苏里尼斯 (Pyrus ussuriensis) (马克西姆) 是一个古老的植物. 是一种全球种植的水果作物,具有食品和功能产品的潜力.
- 关于应用LC-MS相关反应方法来选这种物种中的活性化合物的研究有限.
- 这项研究解决了对高效分析技术的需求,以表征P. ussuriensis代谢物.
研究的目的:
- 开发和验证一种超高性能液体色谱-喷射电离-并联质谱法 (UHPLC-ESI-MS/MS) 方法,用于对代谢物进行分析.
- 为了识别和量化P. ussuriensis提取物中的代谢物.
- 使用在线UHPLC-DPPH测定选抗氧化剂成分,并评估药物相似性.
主要方法:
- 使用光谱匹配与公共数据库对代谢物进行注释.
- 在水和乙醇提取物中确定代谢物的量化.
- 在线UHPLC与2,2-二-1-皮克里利德拉齐尔 (DPPH) 试验相结合,用于抗氧化能力的确定.
- 用于药物相似性评估的药理动力学 (ADME) 评估.
主要成果:
- 在P. ussuriensis提取物中成功识别和量化了各种代谢物.
- 在UHPLC-MS-DPPH测定有效地确定了抗氧化成分.
- 评估了抗氧化干扰和药物相似性.
- 开发的方法在表征活性化合物方面表现出高效率.
结论:
- 在线UHPLC-MS-DPPH方法是植物提取物中抗氧化成分快速表征的强大工具.
- 皮鲁斯乌苏里南斯 (Pyrus ussuriensis) 由于其已识别的抗氧化化合物,具有显著的健康益处潜力.
- 这项研究为未来对P. ussuriensis和类似药用植物的研究提供了有价值的分析框架.
关键词:
ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME ADME在 LC-DPPH 中.这就是LC-MS.皮鲁斯乌苏里南斯 (Pyrus ussuriensis) 是一个古老的植物.抗氧化剂 抗氧化剂是一种抗氧化剂.代谢产物的代谢产物分子网络是分子网络.更多相关视频
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