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优化INFOGEST消化调节以使用Caco-2细胞模型对营养素生物可用性的可靠体外评估
Giulia Camporesi1, Carlo Canzian2, Alessandra Bordoni2
1Interdepartmental Centre for Industrial Agri-Food Research (CIRI), University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy.
Nutrients
|January 28, 2026
概括
使用3kDa膜的超过有效地保持了消化食品中的肠道细胞活力和屏障功能. 这种方法通过改善消化器与肠道模型的兼容性来提高体外生物可用性研究的可靠性.
科学领域:
- 营养科学 营养科学
- 在体外消化模型中的消化模型
- 细胞生物学 细胞生物学
背景情况:
- 营养物质生物可用性的体外评估通常使用胃肠道消化模型与肠道细胞系统相结合.
- 像INFOGEST这样的静态消化协议可能会损害上皮质屏障的完整性,限制研究可重现性.
- 需要标准化的方法来准备消化剂以进行可靠的体外试验.
研究的目的:
- 为了比较五种消化调节策略,以保持肠道上皮细胞活力和屏障功能.
- 确定最优的方法来准备INFOGEST消化剂,用于肠道细胞模型.
- 为了确保生理上相关的度和可重复的暴露条件.
主要方法:
- 酸化,离心,快速冷和超的系统比较 (10kDa和3kDa的MWCO).
- 评估INFOGEST从酸奶,饼干和头鱼中的消化物.
- 评估Caco-2细胞活力 (MTT测定) 和屏障完整性 (TEER测量).
主要成果:
- 3kDa超过在 1:10 稀释下,在所有食物矩阵中始终保持了表皮活力和屏障完整性.
- 其他调节方法在维持可接受的细胞活力的情况下,未能维持体电阻 (TEER).
- 食物矩阵效应影响了较低稀释的结果,需要对矩阵进行特定评估.
结论:
- 3kDa超是一种有效的,最少侵入性的策略,用于改善INFOGEST消化器与肠道细胞模型的兼容性.
- 这种方法使得可重现的暴露条件能够保持上皮质完整性.
- 这些发现支持更可靠的体外生物利用性评估和营养研究中的方法标准化.
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