Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Diffusion01:12

Diffusion

217.9K
Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
217.9K
Free-Radical Chain Reaction and Polymerization of Alkenes02:35

Free-Radical Chain Reaction and Polymerization of Alkenes

9.5K
The conversion of alkenes to macromolecules called polymers is a reaction of high commercial importance. The structure of the polymer is defined by a repeating unit, while the terminal groups are considered insignificant. The average degree of polymerization represents the number of repeating units in the polymer molecule and is denoted by the subscript n.
9.5K
Classification of Titrimetric Analysis Based on Reaction Types01:01

Classification of Titrimetric Analysis Based on Reaction Types

1.7K
Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
Titrations between an acid and a base lead to neutralization reactions that form...
1.7K
Acids, Bases and Neutralization Reactions03:26

Acids, Bases and Neutralization Reactions

63.7K
An acid-base reaction is one in which a hydrogen ion, H+, is transferred from one chemical species to another. Such reactions are of central importance to numerous natural and technological processes, ranging from the chemical transformations within cells or lakes and oceans to the industrial-scale production of fertilizers, pharmaceuticals, and other substances essential to the society.
63.7K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

24.3K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
24.3K
Hybridization of Atomic Orbitals I03:24

Hybridization of Atomic Orbitals I

66.7K
The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
66.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A novel scoring system based on post-radiotherapy changes in pain, performance status, and adverse events to stratify survival in bone metastases.

Scientific reports·2026
Same author

Lobe-Specific Versus Systematic Lymph Node Dissection in Clinical Stage I Non-Small Cell Lung Cancer: A Propensity Score-Matched Analysis Based on the 8th Edition of the TNM Stage Classification.

Thoracic cancer·2026
Same author

Effects of switching from somatropin to somapacitan on thyroid hormone levels in adult growth hormone deficiency: A pilot study.

Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society·2026
Same author

Utility of Postoperative Thyroid Hormone Levels in Predicting the Diagnosis of Adult Growth Hormone Deficiency in Patients Undergoing Surgery for Nonfunctioning Pituitary Neuroendocrine Tumor: A Pilot Study.

International journal of endocrinology·2026
Same author

Acoustofluidic Biosensors.

Micromachines·2026
Same author

Preperitoneal Myelolipoma Occurring after Video-Assisted Thoracoscopic Surgery for Thymoma: A Case Report.

Surgical case reports·2026

相关实验视频

Updated: Jan 29, 2026

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

7.1K

基于高度敏感的混合链反应的miRNA检测技术,使用光探测器修改的miRNA粒子的扩散性分析.

Momoka Nakai1, Yui Watanabe1, Maho Koda1

  • 1Department of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Fukui, Japan.

Sensors (Basel, Switzerland)
|January 28, 2026
PubMed
概括

这项研究引入了一种新的方法,通过分析粒子运动来检测微RNA (miRNA),从而能够在血清中检测出高度敏感的癌症生物标志物. 这种技术为早期癌症诊断提供了潜力.

关键词:
这是一个微型RNA.杂交的连锁反应是混合的.图像分析图像分析可通过照片交叉链接的人造核酸.探测器修改的颗粒颗粒.

更多相关视频

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.9K
Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes
06:32

Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes

Published on: November 17, 2023

1.9K

相关实验视频

Last Updated: Jan 29, 2026

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

7.1K
mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.9K
Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes
06:32

Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes

Published on: November 17, 2023

1.9K

科学领域:

  • 生物标志物发现发现
  • 分子诊断学 分子诊断
  • 纳米技术 纳米技术

背景情况:

  • 微RNAs (miRNAs) 是早期疾病检测的关键生物标志物,特别是在瘤学中.
  • 现有的miRNA检测技术需要进一步提高灵敏度和选择性.
  • 开发新的诊断工具对于及时的疾病干预至关重要.

研究的目的:

  • 通过对布朗运动的图像分析建立一种新的miRNA检测技术.
  • 为了在目标杂交时利用颗粒大小的变化,用于miRNA识别和量化.
  • 提高生物样本中癌症生物标志物的检测灵敏度和特异性.

主要方法:

  • 使用图像分析来追踪光探头修改的miRNA粒子的布朗运动 (扩散性).
  • 使用可光交叉链接的人造核酸 (CNV-D) 探针对目标miRNA进行共价捕获.
  • 整合混合化连锁反应 (HCR) 来放大复杂的尺寸并提高检测灵敏度.

主要成果:

  • 在血清中证明了癌症生物标志物miR-21的高度敏感和特定检测.
  • 实现了异常低的检测极限 (LOD) 1 femtomolar (fM).
  • 在严格的洗条件下在生物样本中证实了该方法的有效性.

结论:

  • 开发的miRNA检测技术显示了早期癌症诊断的巨大潜力.
  • 布朗运动的图像分析为敏感和选择性生物标志物检测提供了一种新的方法.
  • 这项技术可以推进液体活检和个性化医学领域.